Melamine-based Covalent Organic Polymers (MCOPs) as Lipase Nanocarrier for Recyclable Esters Hydrolysis and Transesterification
文献类型：会议论文
| 作者 | Wang, Zhihao; Chen, Ying; Zhao, Jiahe; Gao, Guoliang; Panpipat, Worawan; Cheong, Ling-Zhi; Shen, Cai |
| 出版日期 | 2020 |
| 会议日期 | DEC 07-08, 2019 |
| 关键词 | CANDIDA-RUGOSA LIPASE BETA-GALACTOSIDASE IMMOBILIZATION ENZYME |
| 卷号 | 69 |
| 期号 | 6 |
| DOI | 10.5650/jos.ess20032 |
| 英文摘要 | Present study has successfully synthesized melamine-based covalent organic polymers (MCOPs) and applied it as lipase carrier for recyclable esters hydrolysis and transesterification. The synthesized MCOPs are composed of dense nanosheet structures having a thickness of 3.5 nm. Three immobilization methods namely physical adsorption, cross-linking and carrier activation were employed to prepare the MCOPs-im mobilized CRL. Cross-linked MCOPs-immobilized CRL (41.30 mg protein/g MCOPs) and carrier activated MCOPs-immobilized CRL (33.20 mg protein/g MCOPs) had higher enzyme loading as compared to physical absorb MCOPs-immobilized CRL (29.30 mg protein/g MCOPs). Nevertheless, physical absorb MCOPs-immobilized CRL demonstrated the highest esters hydrolysis (49.85 U) and transesterification (1.04 U) activities. Despite having the highest enzymatic activity, physical absorb MCOPs-immobilized CRL were not able to maintain its catalytic stability with more than 30% decreased in enzymatic activity during consecutive hydrolysis and transesterification activities. Meanwhile, cross-linked MCOPs-immobilized CRL demonstrated highest catalytic stability with highest enzymatic activities at the end of consecutive reactions. All the MCOPs-immobilized CRL can be easily recovered and reused through centrifugation with more than 85% of recovery rate. |
| 学科主题 | Chemistry ; Food Science & Technology |
| ISSN号 | 1345-8957 |
| 源URL | [http://ir.nimte.ac.cn/handle/174433/23301]  |
| 专题 | 会议专题 会议专题_会议论文 |
| 推荐引用方式 GB/T 7714 | Wang, Zhihao,Chen, Ying,Zhao, Jiahe,et al. Melamine-based Covalent Organic Polymers (MCOPs) as Lipase Nanocarrier for Recyclable Esters Hydrolysis and Transesterification[C]. 见:. DEC 07-08, 2019. |
入库方式： OAI收割
来源：宁波材料技术与工程研究所
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