Structural Insights into the Catalytic Mechanism of Synechocystis Magnesium Protoporphyrin IX O-Methyltransferase (ChlM)
文献类型：期刊论文
| 作者 | Chen, Xuemin2; Wang, Xiao2; Feng, Juan2; Chen, Yuhong; Fang, Ying2; Zhao, Shun2; Zhao, Aiguo2; Zhang, Min1; Liu, Lin |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 289期号:37页码:25690-25698 |
| DOI | 10.1074/jbc.M114.584920 |
| 文献子类 | Article |
| 英文摘要 | Magnesium protoporphyrin IX O-methyltransferase (ChlM) catalyzes transfer of the methyl group from S-adenosylmethionine to the carboxyl group of the C13 propionate side chain of magnesium protoporphyrin IX. This reaction is the second committed step in chlorophyll biosynthesis from protoporphyrin IX. Here we report the crystal structures of ChlM from the cyanobacterium Synechocystis sp. PCC 6803 in complex with S-adenosylmethionine and S-adenosylhomocysteine at resolutions of 1.6 and 1.7 angstrom, respectively. The structures illustrate the molecular basis for cofactor and substrate binding and suggest that conformational changes of the two arm regions may modulate binding and release of substrates/products to and from the active site. Tyr-28 and His139 were identified to play essential roles for methyl transfer reaction but are not indispensable for cofactor/substrate binding. Based on these structural and functional findings, a catalytic model is proposed. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | BETHESDA |
| 电子版国际标准刊号 | 1083-351X |
| WOS关键词 | CONTINUOUS SPECTROPHOTOMETRIC ASSAY ; CHELATASE H SUBUNIT ; CRYSTAL-STRUCTURE ; TETRAPYRROLE BIOSYNTHESIS ; ENZYMES ; PROTEIN ; SAM ; PURIFICATION ; EXPRESSION ; PORPHYRIN |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000342130800028 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 资助机构 | National Natural Science Foundation of China [31340044] ; National Basic Research Program of China [2011CBA00901] ; Hundred Talents Program of Chinese Academy of Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27146]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Photosynthesis Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Anhui Univ, Sch Life Sci, Engn Res Ctr Biomed Mat, Hefei 230601, Anhui, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xuemin,Wang, Xiao,Feng, Juan,et al. Structural Insights into the Catalytic Mechanism of Synechocystis Magnesium Protoporphyrin IX O-Methyltransferase (ChlM)[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(37):25690-25698. |
| APA | Chen, Xuemin.,Wang, Xiao.,Feng, Juan.,Chen, Yuhong.,Fang, Ying.,...&Liu, Lin.(2014).Structural Insights into the Catalytic Mechanism of Synechocystis Magnesium Protoporphyrin IX O-Methyltransferase (ChlM).JOURNAL OF BIOLOGICAL CHEMISTRY,289(37),25690-25698. |
| MLA | Chen, Xuemin,et al."Structural Insights into the Catalytic Mechanism of Synechocystis Magnesium Protoporphyrin IX O-Methyltransferase (ChlM)".JOURNAL OF BIOLOGICAL CHEMISTRY 289.37(2014):25690-25698. |
入库方式： OAI收割
来源：植物研究所
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