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Comprehensive quality evaluation of processed Scrophulariae Radix from different regions of China using HPLC coupled with chemometrics methods

文献类型：期刊论文

作者Zhang, Mina1; Chen, Kaixian1,2; Wang, Pan3; Zhang, Liuqiang1; Li, Yiming1
刊名PHYTOCHEMICAL ANALYSIS
出版日期2023-01-26
页码14
关键词chemometrics methods HPLC-DAD multiple simultaneous determination processing methods Scrophulariae Radix
ISSN号0958-0344
DOI10.1002/pca.3209
通讯作者Zhang, Liuqiang(04100217@163.com) ; Li, Yiming(ymlius@163.com)
英文摘要IntroductionScrophulariae Radix (SR) has been extensively used in traditional Chinese medicine (TCM) for thousands of years. However, the processing methods and production areas of Scrophularia ningpoensis have undergone notable historic changes. Thus, their effects on the bioactive constituents of SR still need to be studied further. ObjectivesThis study aimed to establish an objective and comprehensive method to identify the correlation of bioactive constituents of SR with variety, place of origin and processing method for evaluating their qualities. MethodologyAn accurate and rapid high-performance liquid chromatography-diode array detector (HPLC-DAD) method for the simultaneous determination of 11 marker components (aucubin, harpagide, 6-O-methyl-catalpol, harpagoside, verbascoside, isoverbascoside, angoroside C, cinnamic acid, l-tyrosine, l-phenylalanine, and l-tryptophan) was established to evaluate the quality of SR for the first time. In addition, the effects of different production areas and processed methods on the target compounds were studied by analysing 66 batches of SR samples with chemometrics methods, including similarity evaluation of chromatographic fingerprints of TCM, principal component analysis (PCA), and partial least squares-discriminant analysis (PLS-DA). ResultsCompared with "sweating", short-term "steaming" and "slice-drying" could largely preserve the bioactive constituents of SR. When using the model established through PLS-DA, five components were identified as the most significant variables for discrimination. Furthermore, the score plots of PCA and the similarity evaluation revealed that variety had a more notable influence on the quality of SR than the place of origin. ConclusionAn objective approach of HPLC fingerprint coupled with chemometrics analysis and quantitative assessment could be applied to discriminate different processed SR and evaluate the qualities of SR rapidly.
WOS关键词PHENYLPROPANOID GLYCOSIDES ; AMINO-ACIDS
资助项目Three-year Action Plan for the Development of Traditional Chinese Medicine of Shanghai[ZY(2021-2023)-0215] ; National Natural Science Foundation of China[81973458] ; Opening Foundation of National Facility for Translational Medicinal (Shanghai)[TMSK-2021-404]
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种英语
WOS记录号WOS:000921265300001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/303678]  
专题中国科学院上海药物研究所
通讯作者Zhang, Liuqiang; Li, Yiming
作者单位1.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
3.Panan Tradit Chinese Med Ind Innovat & Dev Inst, Hangzhou, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Mina,Chen, Kaixian,Wang, Pan,et al. Comprehensive quality evaluation of processed Scrophulariae Radix from different regions of China using HPLC coupled with chemometrics methods[J]. PHYTOCHEMICAL ANALYSIS,2023:14.
APA Zhang, Mina,Chen, Kaixian,Wang, Pan,Zhang, Liuqiang,&Li, Yiming.(2023).Comprehensive quality evaluation of processed Scrophulariae Radix from different regions of China using HPLC coupled with chemometrics methods.PHYTOCHEMICAL ANALYSIS,14.
MLA Zhang, Mina,et al."Comprehensive quality evaluation of processed Scrophulariae Radix from different regions of China using HPLC coupled with chemometrics methods".PHYTOCHEMICAL ANALYSIS (2023):14.

入库方式： OAI收割

来源：上海药物研究所

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