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Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy

文献类型：期刊论文

作者Ni, Jielei8,9; Cao, Bo7,8,9; Niu, Gang2,3,4,5,6; Chen, Danni7,8,9; Liang, Guotao8,9; Xia, Tingying8,9; Li, Heng1; Xu, Chen8,9; Wang, Jingyu8,9; Zhang, Wanlong8,9
刊名NANOPHOTONICS
出版日期2022
卷号11期号:1页码:53-65
ISSN号2192-8606
关键词confined stochastic motion localization precision single-molecule localization microscopy
DOI10.1515/nanoph-2021-0481
英文摘要Single-molecule localization microscopy (SMLM) plays an irreplaceable role in biological studies, in which nanometer-sized biomolecules are hardly to be resolved due to diffraction limit unless being stochastically activated and accurately located by SMLM. For biological samples preimmobilized for SMLM, most biomolecules are cross-linked and constrained at their immobilizing sites but still expected to undergo confined stochastic motion in regard to their nanometer sizes. However, few lines of direct evidence have been reported about the detectability and influence of confined biomolecule stochastic motion on localization precision in SMLM. Here, we access the potential stochastic motion for each immobilized single biomolecule by calculating the displacements between any two of its localizations at different frames during sequential imaging of Alexa Fluor647-conjugated oligonucleotides. For most molecules, localization displacements are remarkably larger at random frame intervals than at shortest intervals even after sample drift correction, increase with interval times and then saturate, showing that biomolecule stochastic motion is detected and confined around the immobilizing sizes in SMLM. Moreover, localization precision is inversely proportional to confined biomolecule stochasticmotion, whereas it can be deteriorated or improved by enlarging the biomolecules or adding a postcrosslinking step, respectively. Consistently, post-crosslinking of cell samples sparsely stained for tubulin proteins results in a better localization precision. Overall, this study reveals that confined stochastic motion of immobilized biomolecules worsens localization precision in SMLM, and improved localization precision can be achieved via restricting such a motion.
资助项目Guangdong Major Project of Basic and Applied Basic Research[2020B0301030009] ; National Natural Science Foundation of China[31871293] ; National Natural Science Foundation of China[11774242] ; National Natural Science Foundation of China[62005180] ; National Natural Science Foundation of China[62105212] ; Shenzhen Science and Technology Planning Project[JCYJ202103240 93209024] ; Shenzhen Science and Technology Planning Project[JCYJ20210324094200001] ; Shenzhen Science and Technology Planning Project[JCYJ20170817095211560] ; Shenzhen Peacock Plan[KQTD20170330110444030] ; Natural Science Foundation of Guangdong Province[2016A030312010] ; leading talents of Guangdong province[00201505] ; Hefei National Laboratory for Physical Sciences at the Microscale[KF2020009]
WOS研究方向Science & Technology - Other Topics ; Materials Science ; Optics ; Physics
语种英语
出版者WALTER DE GRUYTER GMBH
WOS记录号WOS:000865440400005
源URL[http://119.78.100.204/handle/2XEOYT63/19782]  
专题中国科学院计算技术研究所期刊论文
通讯作者Ni, Yanxiang
作者单位1.Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst TBSI, Shenzhen 518055, Peoples R China
2.Univ Sci & Technol China, Sch Basic Med Sci, Div Life Sci & Med, Hefei Natl Lab Phys Sci Microscale, Hefei 231299, Peoples R China
3.Chinese Acad Sci, Western Inst Comp Technol, Chongqing 400000, Peoples R China
4.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
5.Phil Rivers Technol Ltd, Joint Turing Darwin Lab, Beijing 100190, Peoples R China
6.Phil Rivers Technol, Beijing 100871, Peoples R China
7.Dr Neon Technol Ltd, Shenzhen 518060, Peoples R China
8.Shenzhen Univ, Coll Phys & Optoelect Engn, Coll Elect & Informat Engn, Inst Microscale Optoelect, Shenzhen 518060, Peoples R China
9.Shenzhen Univ, Coll Phys & Optoelect Engn, Coll Elect & Informat Engn, Nanophoton Res Ctr,Shenzhen Key Lab Microscale Op, Shenzhen 518060, Peoples R China
推荐引用方式
GB/T 7714		 Ni, Jielei,Cao, Bo,Niu, Gang,et al. Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy[J]. NANOPHOTONICS,2022,11(1):53-65.
APA Ni, Jielei.,Cao, Bo.,Niu, Gang.,Chen, Danni.,Liang, Guotao.,...&Ni, Yanxiang.(2022).Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy.NANOPHOTONICS,11(1),53-65.
MLA Ni, Jielei,et al."Improved localization precision via restricting confined biomolecule stochastic motion in singlemolecule localization microscopy".NANOPHOTONICS 11.1(2022):53-65.

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来源：计算技术研究所

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