A validated LC-MS/MS method for the quantification of bevacizumab in rat, cynomolgus monkey, and human serum
文献类型：期刊论文
| 作者 | Zhou, Ainan; Yu, Jinghua; Wu, Yali; Xue, Hao; Zhong, Dafang ; Diao, Xingxing
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2023-10-25 |
| 卷号 | 235页码:11 |
| ISSN号 | 0731-7085 |
| 关键词 | Monoclonal antibody Bevacizumab LC-MS MS Surrogate peptide Pharmacokinetics Serum |
| DOI | 10.1016/j.jpba.2023.115590 |
| 通讯作者 | Diao, Xingxing(xxdiao@simm.ac.cn) |
| 英文摘要 | Bevacizumab is a humanized monoclonal antibody used in the treatment of advanced colorectal and non-small cell lung cancer. Our main aim was to establish a simple, economical, and high efficiency liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantifying the content of bevacizumab in various biological fluids (rat, cynomolgus monkey, and human serum). A surrogate peptide of bevacizumab, specifically FTFSLDTSK, was generated through trypsin hydrolysis, and quantified using an isotopically labeled peptide containing two amino acids, FTFSLDTSK[13C6, 15N2]ST, as an internal standard to correct for variations intro-duced during the enzymatic hydrolysis process and any mass spectrometry variabilities. The pre-treatment process included denaturation, disulfide bond reduction and alkylation, trypsin hydrolysis, and termination of the reaction, with a total duration of approximately 2.5-3 h. The results of the methodological validation showed that the linear range in three different biological matrices was 0.2 & mu;g/mL to300 & mu;g/mL, with an LLOQ of 0.2 & mu;g/ mL. The precision and accuracy of the measurements met the required standards. The validated LC-MS/MS method was used to conduct pharmacokinetic analysis in rats administered bevacizumab at a dose of 10 mg/ kg intravenously. |
| WOS关键词 | ANTIBODY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:001052244300001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/306983]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Diao, Xingxing |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ainan,Yu, Jinghua,Wu, Yali,et al. A validated LC-MS/MS method for the quantification of bevacizumab in rat, cynomolgus monkey, and human serum[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2023,235:11. |
| APA | Zhou, Ainan,Yu, Jinghua,Wu, Yali,Xue, Hao,Zhong, Dafang,&Diao, Xingxing.(2023).A validated LC-MS/MS method for the quantification of bevacizumab in rat, cynomolgus monkey, and human serum.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,235,11. |
| MLA | Zhou, Ainan,et al."A validated LC-MS/MS method for the quantification of bevacizumab in rat, cynomolgus monkey, and human serum".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 235(2023):11. |
入库方式： OAI收割
来源：上海药物研究所
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