Structural and mechanistic insights provided by single particle cryo-EM analysis of phosphoinositide 3-kinase (PI3Ka)
文献类型：期刊论文
| 作者 | Vogt, Peter K.1; Hart, Jonathan R.1; Yang, Su1; Zhou, Qingtong2; Yang, Dehua3 ; Wang, Ming-Wei2,4,5
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| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
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| 出版日期 | 2023-09-01 |
| 卷号 | 1878期号:5页码:6 |
| ISSN号 | 0304-419X |
| 关键词 | Phosphoinositide 3-kinase BYL-719 Nanobody CXMS Mutants |
| DOI | 10.1016/j.bbcan.2023.188947 |
| 通讯作者 | Vogt, Peter K.(pkvogt@scripps.edu) |
| 英文摘要 | Recent cryo-electron microscopic (cryo-EM) investigations have succeeded in the analysis of various structural conformations and functional states of PI3K & alpha;, a dimer consisting of the catalytic subunit p110 & alpha; and the regulatory subunit p85 & alpha; of class IA of phosphoinositide 3-kinase. High resolution structures have been obtained of the unliganded and of BYL-719-bound PI3K & alpha;. The latter provides information on excessively flexible domains of p85 & alpha; that are then further analyzed with nanobodies and CXMS (chemical cross-linking, digestion and mass spectrometry). Analysis of p110 & alpha; helical and kinase domain mutations reveals mutant-specific features that can be linked to the gain of function in enzymatic and signaling activities. |
| WOS关键词 | REGULATORY SUBUNIT ; MUTATIONS ; PI3K-ALPHA ; DOMAIN ; ACTIVATION ; P110-ALPHA ; RECEPTOR ; BINDING ; CANCER ; GAIN |
| 资助项目 | U.S. National Cancer Institute[R50CA243899] ; U.S. National Cancer Institute[81872915] ; National Natural Science Foundation of China[82073904] ; National Natural Science Foundation of China[82121005] ; National Natural Science Foundation of China[81973373] ; National Natural Science Foundation of China[21704064] ; National Natural Science Foundation of China[2018ZX09735001] ; National Science and Technology Major Project of China-Key New Drug Creation and Manufacturing Program[2018ZX09711002002-005] ; National Science and Technology Major Project of China-Key New Drug Creation and Manufacturing Program[2018YFA0507000] ; National Key Basic Research Program of China[NNCAS-2017-1-CC] ; Hainan Provincial Major Science and Technology Project[2021ZD0203400] ; Novo Nordisk-Chinese Academy of Sciences Research Fund ; STI2030-Major Project ; [R35 CA197582] ; [ZDKJ2021028] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Oncology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:001057833000001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307037]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Vogt, Peter K. |
| 作者单位 | 1.Scripps Res Inst, Dept Mol Med, 10550 North Torrey Pines Rd, La Jolla, CA 92037 USA 2.Fudan Univ, Sch Basic Med Sci, Dept Pharmacol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Res Ctr Deepsea Bioresources, Sanya 572025, Peoples R China 5.Univ Tokyo, Sch Sci, Dept Chem, Tokyo 1130033, Japan |
| 推荐引用方式 GB/T 7714 | Vogt, Peter K.,Hart, Jonathan R.,Yang, Su,et al. Structural and mechanistic insights provided by single particle cryo-EM analysis of phosphoinositide 3-kinase (PI3Ka)[J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER,2023,1878(5):6. |
| APA | Vogt, Peter K.,Hart, Jonathan R.,Yang, Su,Zhou, Qingtong,Yang, Dehua,&Wang, Ming-Wei.(2023).Structural and mechanistic insights provided by single particle cryo-EM analysis of phosphoinositide 3-kinase (PI3Ka).BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER,1878(5),6. |
| MLA | Vogt, Peter K.,et al."Structural and mechanistic insights provided by single particle cryo-EM analysis of phosphoinositide 3-kinase (PI3Ka)".BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER 1878.5(2023):6. |
入库方式： OAI收割
来源：上海药物研究所
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