Fabricated AIE-Based Probe to Detect the Resistance to Anoikis of Cancer Cells Detached from Tumor Tissue
文献类型：期刊论文
| 作者 | Chang, YN ; Liang, YL; Wang, JY; Chen, ZT; Yan, RY; Chen, K; Li, J; Li, JC; Liang, HJ; Xing, GM
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| 刊名 | CELLS
 |
| 出版日期 | 2022 |
| 卷号 | 11期号:21页码:3478 |
| 关键词 | anoikis aggregation-induced emission probe fluorescence cancer cell |
| DOI | 10.3390/cells11213478 |
| 文献子类 | Article |
| 英文摘要 | (1) Background: Resisting anoikis is a vital and necessary characteristic of malignant cancer cells, but there is no existing quantification method. Herein, a sensitive probe for assessing anoikis resistance of cancer cells detached from the extracellular matrix was developed based on the aggregation-induced emission (AIE) of AIEgens. It has been reported that detached cancer cell endocytose activated integrin clusters, and in the endosome these clusters recruit and activate phosphorylate focal adhesion kinase (pFAK) in the cytoplasm to induce signaling that supports the growth of detached cancer cells. (2) Methods: We established a lost nest cell model of cancer cells and determined their ability to resist anoikis. The colocalization of the activated integrin, pFAK, and endosomes in model cells was observed and calculated. (3) Results: The fluorescence signal intensity of the probe was significantly higher than that of the integrin antibody in the model cells and the fluorescence signal of probe signal was better overlapped with labeled pFAK by fluorescence in endosomes in model cells. (4) Conclusions: We developed a quantitative multi-parametric image analysis program to calculate fluorescent intensity of the probe and antibodies against pFAK and Rab5 in the areas of colocalization. A positive correlation of fluorescence signal intensity between the probe and pFAK on the endosome was observed. Therefore, the probe was used to quantitatively evaluate resisting anoikis of different cancer cell lines under the lost nest condition. |
| 电子版国际标准刊号 | 2073-4409 |
| 语种 | 英语 |
| WOS记录号 | WOS:000883520000001 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/299304]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chang, YN,Liang, YL,Wang, JY,et al. Fabricated AIE-Based Probe to Detect the Resistance to Anoikis of Cancer Cells Detached from Tumor Tissue[J]. CELLS,2022,11(21):3478. |
| APA | Chang, YN.,Liang, YL.,Wang, JY.,Chen, ZT.,Yan, RY.,...&Xing, GM.(2022).Fabricated AIE-Based Probe to Detect the Resistance to Anoikis of Cancer Cells Detached from Tumor Tissue.CELLS,11(21),3478. |
| MLA | Chang, YN,et al."Fabricated AIE-Based Probe to Detect the Resistance to Anoikis of Cancer Cells Detached from Tumor Tissue".CELLS 11.21(2022):3478. |
入库方式： OAI收割
来源：高能物理研究所
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