Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
文献类型：期刊论文
| 作者 | Tao, Yile8,9; Yue, Yang8,9; Qiu, Guangyu8,9; Ji, Zheng7; Spillman, Martin8,9; Gai, Zhibo6; Chen, Qingfa5; Bielecki, Michel4; Huber, Michael3; Trkola, Alexandra3 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2022-02-26 |
| 页码 | 12 |
| 关键词 | SARS-CoV-2 COVID-19 SYBR Green TaqMan probe RT-qPCR |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-022-11822-4 |
| 通讯作者 | Wang, Jing(jing.wang@ifu.baug.ethz.ch) |
| 英文摘要 | The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1 and 2019_nCoV_N3, the performance of the SYBR Green approach was comparable or better than the TaqMan approach, even when considering the newly dominating or emerging variants, including Delta, Eta, Kappa, Lambda, Mu, and Omicron. ORF1ab and 2019_nCoV_N3 were the best combination for sensitive and reliable SARS-CoV-2 molecular diagnostics due to their high sensitivity, specificity, and broad accessibility. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; COVID-19 ; PCR |
| 资助项目 | China Scholarship Council[201906875037] ; China Scholarship Council[201706010410] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000761867000005 |
| 出版者 | SPRINGER |
| 资助机构 | China Scholarship Council |
| 源URL | [http://ir.ieecas.cn/handle/361006/17472]  |
| 专题 | 地球环境研究所_粉尘与环境研究室 第四纪科学与全球变化卓越创新中心 |
| 通讯作者 | Wang, Jing |
| 作者单位 | 1.CAS Ctr Excellence Quaternary Sci & Global Change, Xian 710061, Peoples R China 2.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, State Key Lab Loess & Quaternary Geol, Xian 710061, Peoples R China 3.Univ Zurich, Inst Med Virol, CH-8057 Zurich, Switzerland 4.Univ Zurich, Epidemiol Biostat & Prevent Inst, CH-8091 Zurich, Switzerland 5.Liaocheng Univ, Inst Tissue Engn & Regenerat Med, Liaocheng 252000, Shandong, Peoples R China 6.Univ Zurich, Univ Hosp Zurich, Dept Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland 7.Shaanxi Normal Univ, Sch Geog & Tourism, Xian 710119, Peoples R China 8.Swiss Fed Labs Mat Sci & Technol, Lab Adv Analyt Technol, Empa, CH-8600 Dubendorf, Switzerland 9.Swiss Fed Inst Technol, Inst Environm Engn, CH-8093 Zurich, Switzerland |
| 推荐引用方式 GB/T 7714 | Tao, Yile,Yue, Yang,Qiu, Guangyu,et al. Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2022:12. |
| APA | Tao, Yile.,Yue, Yang.,Qiu, Guangyu.,Ji, Zheng.,Spillman, Martin.,...&Wang, Jing.(2022).Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,12. |
| MLA | Tao, Yile,et al."Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022):12. |
入库方式： OAI收割
来源：地球环境研究所
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