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Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis

文献类型：期刊论文

作者Wang, Ruohan3; Chen, Ling3; Jia, Yaping3; Liu, Liya2; Sun, Liwei3; Liu, Yujun3; Li, Yun3
刊名FRONTIERS IN PLANT SCIENCE
出版日期2022
卷号13
关键词floral thermogenesis high-throughput sequencing alternative respiration cell wall volatile organic compounds
ISSN号1664-462X
DOI10.3389/fpls.2022.955665
文献子类Article
英文摘要Floral thermogenesis is coupled with odor emission in known thermogenic plants. It is widely accepted that elevation in floral temperature can help release of volatile organic compounds (VOCs). However, no information is available about whether floral thermogenesis is associated with VOC biosynthesis. Here, we used RNA-Sequencing (RNA-Seq) to draw a gene expression atlas of floral thermogenesis in Magnolia denudata and captured an upregulation of Alternative Oxidase (AOX) during floral thermogenesis. Western blot analyses also suggested upregulation of AOX during floral thermogenesis. Moreover, oxygen consumption analyses revealed increased activity of the AOX respiration pathway during floral thermogenesis. Using HPLC analyses, we further found that increased AOX respiration substantially promoted production of citric acid by 1.35 folds, which provided fundamental metabolite skeletons for biosynthesis of VOCs. RNA-Seq also showed upregulation of genes regulating lignin catabolism, which was in agreement with in situ Raman chemical imaging of lignin. Taken together, our results suggest the central role of AOX by coupling heat production and VOC biosynthesis in floral thermogenesis of M. denudata.
学科主题Plant Sciences
出版地LAUSANNE
WOS关键词SKUNK CABBAGE ; UNCOUPLING-PROTEIN ; TEMPERATURE REGULATION ; LABEL-FREE ; MITOCHONDRIAL ; POLLINATION ; PATHWAY ; EXPRESSION ; OXIDASE ; SPADIX
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000876754000001
出版者FRONTIERS MEDIA SA
资助机构Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; [2021ZY63] ; [31770201]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28881]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
2.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Key Lab Genet & Breeding Forest Trees & Ornamental, Minist Educ, Beijing, Peoples R China
3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Natl Engn Lab Tree Breeding, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Ruohan,Chen, Ling,Jia, Yaping,et al. Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA Wang, Ruohan.,Chen, Ling.,Jia, Yaping.,Liu, Liya.,Sun, Liwei.,...&Li, Yun.(2022).Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis.FRONTIERS IN PLANT SCIENCE,13.
MLA Wang, Ruohan,et al."Heat production and volatile biosynthesis are linked via alternative respiration in Magnolia denudata during floral thermogenesis".FRONTIERS IN PLANT SCIENCE 13(2022).

入库方式： OAI收割

来源：植物研究所

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