Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods
文献类型：期刊论文
| 作者 | Dai,Haopeng; He,Min; Hu,Guilin; Li,Zhongrong; Al-Romaima,Abdulbaset; Wu,Zhouwei; Liu,Xiaocui; Qiu,Minghua
|
| 刊名 | FOODS
 |
| 出版日期 | 2023 |
| 卷号 | 12期号:18页码:3480 |
| 关键词 | coffee protein peptide ACE inhibitor LC-MS/MS molecular docking ANGIOTENSIN-CONVERTING ENZYME IDENTIFICATION |
| ISSN号 | 2304-8158 |
| DOI | 10.3390/foods12183480 |
| 英文摘要 | Inhibition of angiotensin-I converting enzyme (ACE) is an important means of treating hypertension since it plays an important regulatory function in the renin-angiotensin system. The aim of this study was to investigate the ACE inhibitory effect of bioactive peptides from green coffee beans using in silico and in vitro methods. Alcalase and thermolysin were employed to hydrolyze protein extract from coffee beans. Bioactive peptides were identified by LC-MS/MS analysis coupled with database searching. The potential bioactivities of peptides were predicted by in silico screening, among which five novel peptides may have ACE inhibitory activity. In vitro assay was carried out to determine the ACE inhibitory degree. Two peptides (IIPNEVY, ITPPVMLPP) were obtained with IC50 values of 57.54 and 40.37 & mu;M, respectively. Furthermore, it was found that two inhibitors bound to the receptor protein on similar sites near the S1 active pocket of ACE to form stable enzyme-peptide complexes through molecular docking, and the Lineweaver-Burk plot showed that IIPNEVY was a noncompetitive inhibitor, and ITPPVMLPP was suggested to be a mixed-type inhibitor. Our study demonstrated that two peptides isolated from coffee have potential applications as antihypertensive agents. |
| WOS记录号 | WOS:001073981100001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75009]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Dai,Haopeng,He,Min,Hu,Guilin,et al. Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods[J]. FOODS,2023,12(18):3480. |
| APA | Dai,Haopeng.,He,Min.,Hu,Guilin.,Li,Zhongrong.,Al-Romaima,Abdulbaset.,...&Qiu,Minghua.(2023).Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods.FOODS,12(18),3480. |
| MLA | Dai,Haopeng,et al."Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods".FOODS 12.18(2023):3480. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。