Pharmacokinetics and metabolite identification of 23-hydroxybetulinic acid in rats by using liquid chromatography-mass spectrometry method
文献类型：期刊论文
| 作者 | Xue, Mingzhen2,3,4; Yang, Cheng1; Huang, Wensi3; He, Yifei3; Yang, Chen3; Xue, Yaru3; Zheng, Yuandong3; Diao, Xingxing1,3; Wang, Xiachang2,4 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2024-02-15 |
| 卷号 | 1234页码:10 |
| 关键词 | 23-hydroxybetulinic acid Pharmacokinetics Metabolism UPLC-MS LC-MS/MS |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2024.124016 |
| 通讯作者 | Zheng, Yuandong(ydzheng@simm.ac.cn) ; Diao, Xingxing(xxdiao@simm.ac.cn) ; Wang, Xiachang(xiachangwang@njucm.edu.cn) |
| 英文摘要 | 23-hydroxybetulinic acid (23 -HA), a main bioactive component isolated from Pulsatilla chinensis (Bunge) Regel, exhibits various pharmacological activities, such as antimelanoma, antileukemia, anti -colon cancer, and antihepatotoxicity. Although the main active ingredient anemoside B4 (AB4) from this plant has been well studied, research on its active metabolite 23 -HA is limited. In the present study, a validated HPLC-QQQ-MS/MS method was established for the quantification of 23 -HA in rat plasma. Pharmacokinetics analysis showed that the absorption and elimination of 23 -HA in rats were rapid, with an oral bioavailability as 12.9 %. After oral administration with 50 mg/kg 23 -HA for SD rats, the plasma, urine, feces, and bile samples were collected and analyzed by UPLC-Q Exactive Plus MS and HPLC-QQQ-MS/MS. Seventeen metabolites of 23 -HA were identified, and its major metabolic pathways included oxidation, hydration, sulfation, and glucuronidation. This study highlights the first detailed investigation of 23-HA's pharmacokinetics in rats along with its metabolism in vivo, and will provide robust evidence for further research and clinical application of 23 -HA. |
| 资助项目 | National Key R&D Program of China[2022YFF1202600] ; Na-tional Natural Science Foundation of China[82204585] ; Key Technologies R&D Program of Guangdong Province[2023B1111030004] ; Jiangsu Provincial Innovation and Entrepreneurship Talent Plan[202010528] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001173345700001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310644]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zheng, Yuandong; Diao, Xingxing; Wang, Xiachang |
| 作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 2.Quanzhou Normal Univ, Coll Oceanol & Food Sci, Quanzhou 362000, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China 4.Nanjing Univ Chinese Med, Jiangsu Key Lab Funct Subst Chinese Med, Nanjing 210023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Mingzhen,Yang, Cheng,Huang, Wensi,et al. Pharmacokinetics and metabolite identification of 23-hydroxybetulinic acid in rats by using liquid chromatography-mass spectrometry method[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2024,1234:10. |
| APA | Xue, Mingzhen.,Yang, Cheng.,Huang, Wensi.,He, Yifei.,Yang, Chen.,...&Wang, Xiachang.(2024).Pharmacokinetics and metabolite identification of 23-hydroxybetulinic acid in rats by using liquid chromatography-mass spectrometry method.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1234,10. |
| MLA | Xue, Mingzhen,et al."Pharmacokinetics and metabolite identification of 23-hydroxybetulinic acid in rats by using liquid chromatography-mass spectrometry method".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1234(2024):10. |
入库方式： OAI收割
来源：上海药物研究所
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