Digital colloid-enhanced Raman spectroscopy for the pharmacokinetic detection of bioorthogonal drugs
文献类型：期刊论文
| 作者 | Bi, Xinyuan6; He, Zhicheng6; Luo, Zhewen6; Huang, Wensi5,6; Diao, Xingxing4,5; Ye, Jian1,2,3,6 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2024-08-02 |
| 页码 | 11 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/d4sc02553a |
| 通讯作者 | Ye, Jian() |
| 英文摘要 | Bioorthogonal drug molecules are currently gaining prominence for their excellent efficacy, safety and metabolic stability. Pharmacokinetic study is critical for understanding their mechanisms and guiding pharmacotherapy, which is primarily performed with liquid chromatography-mass spectrometry as the gold standard. For broader and more efficient applications in clinics and fundamental research, further advancements are especially desired in cheap and portable instrumentation as well as rapid and tractable pretreatment procedures. Surface-enhanced Raman spectroscopy (SERS) is capable of label-free detection of various molecules based on the spectral signatures with high sensitivity even down to a single-molecule level. But limited by irreproducibility at low concentrations and spectral interference in complex biofluids, SERS hasn't been widely applied for pharmacokinetics, especially in live animals. In this work, we propose a new method to quantify bioorthogonal drug molecules with signatures at the spectral silent region (SR) by the digital colloid-enhanced Raman spectroscopy (dCERS) technique. This method was first validated using 4-mercaptobenzonitrile in a mixture of analogous molecules, exhibiting reliable and specific identification capability based on the unique SR signature and Poisson-determined quantification accuracy. We further developed a single-step serum pretreatment method and successfully profiled the pharmacokinetic behavior of an anticancer drug, erlotinib, from animal studies. In a word, this method, superior in sensitivity, controllable accuracy, minimal background interference and facile pretreatment and measurement, promises diverse applications in fundamental studies and clinical tests of bioorthogonal drug molecules. Bioorthogonal drug molecules with the Raman signatures at the spectral silent region have been quantified with controllable reproducibility by digital colloid-enhanced Raman spectroscopy for rapid pharmacokinetic detection in live animals. |
| WOS关键词 | SINGLE-MOLECULE ; SILVER NANOPARTICLES ; SERS ; PLASMA ; ADSORPTION ; IDENTIFICATION ; METABOLISM ; ERLOTINIB ; PROTEINS ; MS/MS |
| 资助项目 | National Natural Science Foundation of China[82272054] ; Shanghai Key Laboratory of Gynecologic Oncology ; Shanghai Jiao Tong University[YG2024LC09] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001288809000001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/312676]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ye, Jian |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Peoples Hosp 6, Sch Med, Shanghai 200233, Peoples R China 2.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, Shanghai Key Lab Gynecol Oncol, Shanghai 200127, Peoples R China 3.Shanghai Jiao Tong Univ, Inst Med Robot, Shanghai 200240, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Biomed Engn, State Key Lab Syst Med Canc, Shanghai 200030, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, Xinyuan,He, Zhicheng,Luo, Zhewen,et al. Digital colloid-enhanced Raman spectroscopy for the pharmacokinetic detection of bioorthogonal drugs[J]. CHEMICAL SCIENCE,2024:11. |
| APA | Bi, Xinyuan,He, Zhicheng,Luo, Zhewen,Huang, Wensi,Diao, Xingxing,&Ye, Jian.(2024).Digital colloid-enhanced Raman spectroscopy for the pharmacokinetic detection of bioorthogonal drugs.CHEMICAL SCIENCE,11. |
| MLA | Bi, Xinyuan,et al."Digital colloid-enhanced Raman spectroscopy for the pharmacokinetic detection of bioorthogonal drugs".CHEMICAL SCIENCE (2024):11. |
入库方式： OAI收割
来源：上海药物研究所
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