Miamiensis avidus, a Novel Scuticociliate Pathogen Isolated and Identified from Cultured Large Yellow Croaker (Larimichthys crocea)
文献类型：期刊论文
| 作者 | Lin, Nengfeng1; Pan, Ying1; Zhan, Zifeng2; Xu, Binfu1; Gong, Hui1,3; Zeng, Hong4 |
| 刊名 | PATHOGENS
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 13期号:8页码:12 |
| 关键词 | L. crocea Scuticociliatosis Miamiensis avidus alpha-tubulin indirect immunofluorescence molecular identification |
| DOI | 10.3390/pathogens13080618 |
| 通讯作者 | Zeng, Hong(zhbio@fjnu.edu.cn) |
| 英文摘要 | Scuticociliates are recognized as the causative agents of mass mortalities in certain cultured marine fishes, resulting in enormous economic losses. This study aimed to investigate a fatal infection caused by scuticociliates in farmed large yellow croaker (Larimichthys crocea) in Fujian province, China. Microscopic examinations of focal organs, including the brain, eyes, gills, and skin, revealed the presence of parasites. Active masses of scuticociliates were observed in these organs, and the ciliates were subsequently isolated and maintained in vitro. An immersion challenge experiment revealed that L. crocea experienced cumulative mortalities reaching 73% within 7 d upon exposure to 1.0 x 10(4 )ciliates mL(-1). Based on the microscopic and PCR testing of infected fishes, the brain was comprehensively inferred as the main infection organ for the isolated strain. Microscopic and submicroscopic observations of the isolated scuticociliate, coupled with cortical ciliature patterns revealed through alpha-tubulin indirect immunofluorescence techniques, identified these scuticociliates as Miamiensis avidus. The sequencing of two genetic markers (small subunit ribosomal RNA, SSU rRNA and cytochrome c oxidase subunit I, COI) further confirmed that the isolated strains exhibited the highest sequence similarity to most M. avidus sequences in GenBank. However, significant differences in SSU sequences compared to the M. avidus strain Ma/2, and the lack of published COI and ITS (internal transcribed spacer) sequences for Ma/2, indicate the need for further molecular data to resolve whether there are potential cryptic species within the M. avidus complex. |
| WOS关键词 | PHILASTERIDES-DICENTRARCHI CILIOPHORA ; FLOUNDER PARALICHTHYS-OLIVACEUS ; TURBOT SCOPHTHALMUS-MAXIMUS ; PSEUDOCOHNILEMBUS-PERSALINUS ; URONEMA-MARINUM ; IN-VITRO ; N-SP ; INFECTION ; PARASITE ; TAXONOMY |
| 资助项目 | National Key Research and Development Program of China ; Special Fund for Public-interest Scientific Institutions of Science and Technology Plan Projects of Fujian Province[2019R1027-1] ; General Projects of Fujian Academy of Agricultural Science[AC2017-1] ; Scientific Research Innovation Program Xiyuanjiang River Scholarship of College of Life Sciences, Fujian Normal University ; [2022YFD2401002] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001306710000001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198275]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Zeng, Hong |
| 作者单位 | 1.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Peoples R China 2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China 3.State Key Lab Mariculture Breeding, Ningde 352115, Peoples R China 4.Fujian Normal Univ, Coll Life Sci, Publ Serv Platform Industrializat Dev Technol Mari, Fuzhou 350117, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Nengfeng,Pan, Ying,Zhan, Zifeng,et al. Miamiensis avidus, a Novel Scuticociliate Pathogen Isolated and Identified from Cultured Large Yellow Croaker (Larimichthys crocea)[J]. PATHOGENS,2024,13(8):12. |
| APA | Lin, Nengfeng,Pan, Ying,Zhan, Zifeng,Xu, Binfu,Gong, Hui,&Zeng, Hong.(2024).Miamiensis avidus, a Novel Scuticociliate Pathogen Isolated and Identified from Cultured Large Yellow Croaker (Larimichthys crocea).PATHOGENS,13(8),12. |
| MLA | Lin, Nengfeng,et al."Miamiensis avidus, a Novel Scuticociliate Pathogen Isolated and Identified from Cultured Large Yellow Croaker (Larimichthys crocea)".PATHOGENS 13.8(2024):12. |
入库方式： OAI收割
来源：海洋研究所
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