Utility of quantitative whole-body autoradiography (QWBA) and oxidative combustion (OC) analysis in the assessment of tissue distribution of [14C]Mefuparib (CVL218) in SD and LE rats
文献类型：期刊论文
| 作者 | Li, Xinmei4; Wang, Feiyu2,3; Zhang, Xinyue2,3; Shen, Xiaokun1; Yang, Chunhao2 ; Zheng, Yuandong2,4; Diao, Xingxing2,3,4
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| 刊名 | PLOS ONE
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| 出版日期 | 2024-12-05 |
| 卷号 | 19期号:12页码:13 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0315223 |
| 通讯作者 | Zheng, Yuandong(ydzheng@simm.ac.cn) ; Diao, Xingxing(xxdiao@simm.ac.cn) |
| 英文摘要 | Background In tissue distribution studies of radiopharmaceuticals, quantitative whole-body autoradiography (QWBA) and oxidative combustion (OC) analysis are the two important methods that have not been compared using the same drug. Sprague-Dawley (SD) and Long-Evans (LE) rats, both of which are commonly used rodents in tissue distribution studies, have also not been compared using the same drug. Comparative studies are important for aiding the selection of appropriate experimental methods and animals. Methods To evaluate the tissue distribution of [C-14]Mefuparib (CVL218) in rats and assess its clinical safety, QWBA and OC analysis were used. The differences between the two methods were noted. Comparisons between the tissue distribution results of LE and SD rats were also done. Results The QWBA and OC distribution analysis showed that [C-14]CVL218-related radioactivity could be distributed in 19 tissues. For 89.47% of the tissues, no significant differences were noted between the two methods. There were also no differences in the pharmacokinetics data for plasma and brain homogenates between LE and SD rats. However, the pharmacokinetics data for liver and kidney homogenates were seven-fold higher in LE rats than in SD ones. Conclusions Both the OC and QWBA methods revealed that [C-14]CVL218 could be widely distributed in the tissues of rats. The OC had a lower limit of quantification while QWBA provided a more comprehensive analysis of [C-14]CVL218 distribution. More safety was associated with using LE rat data to estimate the dosimetry of [C-14]CVL218 for the whole-body, for human radiolabeled mass balance studies. |
| WOS关键词 | METABOLITES |
| 资助项目 | National Natural Science Foundation of China[82373938] ; Yunnan Provincial Department of Education Scientific Research Fund[2024J0310] ; Talent Trusteeship Program of Fuwai Yunnan Hospital[2024RCTJ-QN005] ; Kunming Medical University Alliance Special Project[202101AC070477] ; Yunnan Provincial Clinical Research Center for Cardiovascular Diseases[202102AA310002] ; Research and Application of Epidemiology, Pathogenesis, Diagnosis and Treatment of Cardiovascular Diseases in High Altitude of Yunnan Province Project[202103AC100004] ; Key Technology Research and Device Development Project for Innovative Diagnosis and Treatment of Structural Heart Disease in the Southwest Plateau Region[202302AA310045] ; National Clinical Research Center for Cardiovascular Diseases -Yunnan Provincial Sub-center |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001383041800211 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315576]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zheng, Yuandong; Diao, Xingxing |
| 作者单位 | 1.Convalife Shanghai Co Ltd, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Medica, Nanjing, Peoples R China 4.Kunming Med Univ, Chinese Acad Med Sci, Fuwai Yunnan Hosp, Affiliated Cardiovasc Hosp,Dept Pharm, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xinmei,Wang, Feiyu,Zhang, Xinyue,et al. Utility of quantitative whole-body autoradiography (QWBA) and oxidative combustion (OC) analysis in the assessment of tissue distribution of [14C]Mefuparib (CVL218) in SD and LE rats[J]. PLOS ONE,2024,19(12):13. |
| APA | Li, Xinmei.,Wang, Feiyu.,Zhang, Xinyue.,Shen, Xiaokun.,Yang, Chunhao.,...&Diao, Xingxing.(2024).Utility of quantitative whole-body autoradiography (QWBA) and oxidative combustion (OC) analysis in the assessment of tissue distribution of [14C]Mefuparib (CVL218) in SD and LE rats.PLOS ONE,19(12),13. |
| MLA | Li, Xinmei,et al."Utility of quantitative whole-body autoradiography (QWBA) and oxidative combustion (OC) analysis in the assessment of tissue distribution of [14C]Mefuparib (CVL218) in SD and LE rats".PLOS ONE 19.12(2024):13. |
入库方式： OAI收割
来源：上海药物研究所
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