AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip
文献类型：期刊论文
| 作者 | Yang, Zhicheng2,4; Jin, Kai3; Chen, Yimin2,4; Liu, Qian2; Chen, Hongxu5; Hu, Siyi3; Wang, Yuqiu2; Pan, Zilu1; Feng, Fang1; Shi, Mude6 |
| 刊名 | JACS AU
 |
| 出版日期 | 2024-03-26 |
| 卷号 | 4期号:5页码:1811-1823 |
| 关键词 | single-cell proteomics active matrix digital microfluidics(AM-DMF) integrated microfluidic platform massspectrometry cellular heterogeneity |
| DOI | 10.1021/jacsau.4c00027 |
| 文献子类 | Article |
| 英文摘要 | Single-cell proteomics offers unparalleled insights into cellular diversity and molecular mechanisms, enabling a deeper understanding of complex biological processes at the individual cell level. Here, we develop an integrated sample processing on an active-matrix digital microfluidic chip for single-cell proteomics (AM-DMF-SCP). Employing the AM-DMF-SCP approach and data-independent acquisition (DIA), we identify an average of 2258 protein groups in single HeLa cells within 15 min of the liquid chromatography gradient. We performed comparative analyses of three tumor cell lines: HeLa, A549, and HepG2, and machine learning was utilized to identify the unique features of these cell lines. Applying the AM-DMF-SCP to characterize the proteomes of a third-generation EGFR inhibitor, ASK120067-resistant cells (67R) and their parental NCI-H1975 cells, we observed a potential correlation between elevated VIM expression and 67R resistance, which is consistent with the findings from bulk sample analyses. These results suggest that AM-DMF-SCP is an automated, robust, and sensitive platform for single-cell proteomics and demonstrate the potential for providing valuable insights into cellular mechanisms. |
| WOS关键词 | SAMPLE PREPARATION ; MASS-SPECTROMETRY ; HETEROGENEITY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001191204800001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/317019]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xie, Hua; Ma, Hanbin; Zhou, Hu |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou 215163, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China 6.Guangdong ACXEL Micro & Nano Tech Co Ltd, Foshan 528000, Guangdong, Peoples R China 7.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 8.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Zhicheng,Jin, Kai,Chen, Yimin,et al. AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip[J]. JACS AU,2024,4(5):1811-1823. |
| APA | Yang, Zhicheng.,Jin, Kai.,Chen, Yimin.,Liu, Qian.,Chen, Hongxu.,...&Zhou, Hu.(2024).AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip.JACS AU,4(5),1811-1823. |
| MLA | Yang, Zhicheng,et al."AM-DMF-SCP: Integrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip".JACS AU 4.5(2024):1811-1823. |
入库方式： OAI收割
来源：上海药物研究所
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