Accurate isotopic analysis of nitrate and nitrite in freshwater: integration of ion exchange and bacterial denitrification methods
文献类型：期刊论文
| 作者 | Hu, Jing3; Yao, Shao-jie2; Feng, Miao-miao2; Li, Yu-liang2; Ren, Hao-yu2; Yang, Kai-li1; Liu, Wei-guo3 |
| 刊名 | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
 |
| 出版日期 | 2026-03-12 |
| 页码 | 10 |
| ISSN号 | 0267-9477 |
| DOI | 10.1039/d5ja00363f |
| 通讯作者 | Li, Yu-liang(yulianglee@hotmail.com) ; Liu, Wei-guo(iuwg@loess.llqg.ac.cn) |
| 英文摘要 | The bacterial denitrification method (Pseudomonas aureofaciens) is a widely used pretreatment technique for nitrogen and oxygen isotope analysis of nitrate (NO3-) and nitrite (NO2-), but concurrent reduction of both species (NO3- and NO2-) to N2O obscures their individual isotopic signals. This study developed an integrated ion-exchange (AG1-X8 resin) and denitrification for the methods, achieving quantitative NO3-/NO2- separation (0.25 mol per L KCl for NO2- and 0.5 mol per L HCl for NO3- at 0.5-3 mL min-1) and high-precision isotopic analysis (delta 15N: SD <= 0.2 parts per thousand and delta 18O: SD <= 0.3 parts per thousand for both NO2- and NO3- at >= 0.054 mmol L-1). This method provides a safe, stable, and high-accuracy approach for separation of NO3-/NO2- in high-NO2- freshwater systems. In addition, to effectively address the instability of NO2- during storage, samples can be preserved immediately upon collection by adsorption onto resin. In conclusion, this study has established an efficient and accurate pretreatment method for separating NO2- and NO3- and determining their individual N and O isotopes in natural freshwater. This method provides a reliable analytical technique for in-depth investigation of nitrogen transformation mechanisms, especially in high NO2- environments, using NO2- isotopic signatures as an indicator. |
| WOS关键词 | CHEMICAL CONVERSION ; OXYGEN ISOTOPES ; NITROUS-OXIDE ; DELTA-N-15 ; COLLECTION ; DELTA-O-18 ; AMMONIUM ; SEAWATER ; RATIOS ; N-15 |
| 资助项目 | Chinese Academy of Sciences[2024g103] ; National Natural Science Foundation of China[42377261] ; National Natural Science Foundation of China[42207300] |
| WOS研究方向 | Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:001733276800001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Chinese Academy of Sciences ; National Natural Science Foundation of China |
| 源URL | [http://ir.ieecas.cn/handle/361006/17824]  |
| 专题 | 地球环境研究所_现代环境研究室 |
| 通讯作者 | Li, Yu-liang; Liu, Wei-guo |
| 作者单位 | 1.Xian Inst Innovat Earth Environm Res, Xian 710060, Shaanxi, Peoples R China 2.Changan Univ, Key Lab Subsurface Hydrol & Ecol Effects Arid Reg, Sch Water & Environm,Minist Water Resources, Key Lab Ecohydrol & Water Secur Arid & Semiarid Re, Xian 710064, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Earth Environm, State Key Lab Loess Sci, Xian 710061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Jing,Yao, Shao-jie,Feng, Miao-miao,et al. Accurate isotopic analysis of nitrate and nitrite in freshwater: integration of ion exchange and bacterial denitrification methods[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2026:10. |
| APA | Hu, Jing.,Yao, Shao-jie.,Feng, Miao-miao.,Li, Yu-liang.,Ren, Hao-yu.,...&Liu, Wei-guo.(2026).Accurate isotopic analysis of nitrate and nitrite in freshwater: integration of ion exchange and bacterial denitrification methods.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,10. |
| MLA | Hu, Jing,et al."Accurate isotopic analysis of nitrate and nitrite in freshwater: integration of ion exchange and bacterial denitrification methods".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY (2026):10. |
入库方式： OAI收割
来源：地球环境研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。