中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
Membrane fusion deficits of acutely isolated synaptic vesicles from the brains of depression-like mice

文献类型：期刊论文

作者Zhao, Xuanzhu6,7; Shah, Pir Tariq6,7; Feng, Lijin6; Song, Siqi6,7; Feng, Yazhuo6,7; Wu, Chengjun5; Liu, Rong4; Wang, Yujun2,3,6; Zhang, Sheng1; Wu, Zhenyong2,3,6
刊名NEUROSCIENCE LETTERS
出版日期2025-10-25
卷号866页码:8
关键词Depression Synaptic vesicles Membrane fusion Neurotransmitter release
ISSN号0304-3940
DOI10.1016/j.neulet.2025.138366
通讯作者Wang, Yujun(yjwang@simm.ac.cn) ; Zhang, Sheng() ; Wu, Zhenyong(wuzhenyong@simm.ac.cn)
英文摘要Depression is a common and debilitating psychiatric disorder increasingly associated with presynaptic dysfunction, particularly impairments in synaptic vesicle (SV)-mediated neurotransmitter release, yet its underlying neurobiological mechanisms remain largely unresolved. In this study, we investigated SV morphology and fusion capability in a chronic variable stress (CVS)-induced mouse model of depression. To enable this analysis, we established an optimized protocol integrating differential centrifugation, density gradient ultracentrifugation, and size exclusion chromatography to isolate high-purity SVs from mouse brain tissue. Behavioral analyses confirmed the depression-like phenotype in CVS mice, characterized by anhedonia, anxiety-like behavior and behavioral despair. Although SV morphology, as characterized by TEM, appeared largely preserved, reconstituted vesicle fusion assays demonstrated a significant decline in their fusion capacity, suggesting impaired neurotransmitter release. These findings provide direct evidence of functional SV alternations in depression and underscore the key role of presynaptic dysfunction in its pathophysiology. The optimized SV isolation workflow developed in this study offers a valuable tool for investigating presynaptic mechanisms in neuropsychiatric disorders.
WOS关键词PREFRONTAL CORTEX ; DISORDER
资助项目Key R&D Program of Shandong Province, China[2024CXPT029] ; National Natural Science Foundation of China[22372024] ; Taishan Scholars Program[2023000027] ; Fundamental Research Funds for the Central Universities[DUT24ZD132] ; The Excellence Co-innovation Program International Exchange Fund Project[DUTIO-ZG-202505] ; Special Supporting Funds for Leading Talents at or Above the Provincial Level in Yantai City[2023000090] ; National Natural Science Foundation of China[22372024]
WOS研究方向Neurosciences & Neurology
语种英语
WOS记录号WOS:001564356700001
出版者ELSEVIER IRELAND LTD
源URL[http://119.78.100.183/handle/2S10ELR8/321378]  
专题中国科学院上海药物研究所
通讯作者Wang, Yujun; Zhang, Sheng; Wu, Zhenyong
作者单位1.Dalian Univ Technol, Frontier Sci Ctr Smart Mat, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
4.Dalian Univ Technol, Sch Biomed Engn, Liaoning Key Lab IC & BME Syst, 2 Linggong Rd, Dalian 116024, Liaoning, Peoples R China
5.Univ Hlth & Rehabil Sci, Qingdao Cent Hosp, Sch Hlth & Life Sci, Qingdao 266113, Peoples R China
6.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
7.Dalian Univ Technol, Fac Med, Dalian 116024, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Xuanzhu,Shah, Pir Tariq,Feng, Lijin,et al. Membrane fusion deficits of acutely isolated synaptic vesicles from the brains of depression-like mice[J]. NEUROSCIENCE LETTERS,2025,866:8.
APA Zhao, Xuanzhu.,Shah, Pir Tariq.,Feng, Lijin.,Song, Siqi.,Feng, Yazhuo.,...&Wu, Zhenyong.(2025).Membrane fusion deficits of acutely isolated synaptic vesicles from the brains of depression-like mice.NEUROSCIENCE LETTERS,866,8.
MLA Zhao, Xuanzhu,et al."Membrane fusion deficits of acutely isolated synaptic vesicles from the brains of depression-like mice".NEUROSCIENCE LETTERS 866(2025):8.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。