Exploring the regulatory mechanism of CCNA2 in colorectal cancer: Insights from multiomics and experimental analysis
文献类型：期刊论文
| 作者 | Lei, Xinyi11,12; Qiu, Lanying10; Chen, Qiang9; Liao, Lan8; Yu, Pengfei12; Wu, Wenjie7; Zhu, Zhengyang6; Li, Chunying5; Lin, Gang3,4; Zhuang, Zirui3,4 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2025-08-01 |
| 卷号 | 301期号:8页码:16 |
| DOI | 10.1016/j.jbc.2025.110216 |
| 通讯作者 | Wang, Cunchuan(twcc2015@163.com) ; Du, Yian(duya@zjcc.org.cn) |
| 英文摘要 | Colorectal cancer (CRC) is the third-most common cancer and the second leading of cancer-related deaths worldwide. The underlying regulatory mechanism of cyclin A2 (CCNA2) in CRC was explored through multiomics and experimental analyses, thus facilitating diagnosis, therapy, and prognosis. GSE9348 and GSE110223 were extracted from Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified via GEO2R. CCNA2, a core gene for CRC, was screened out from the protein-protein interaction network constructed by differentially expressed genes. Its diagnostic, prognostic, and therapeutic value was evaluated in Gene Expression Omnibus, The Cancer Genome Atlas, Human Protein Atlas, and Drug-Gene interaction database via transcriptomics, proteomics, and pharmacogenomics. The correlation between CCNA2 and immune infil-tration was determined in Tumor Immune Estimation Resource by immunomics. Transcription factor-mRNA and miRNA-mRNA networks for CCNA2 were constructed in miRnet and miRDB via transcriptomics. The role and regulatory mechanism of CCNA2 in CRC were investigated both in vitro and in vivo. CCNA2 showed excellent diagnostic, therapeutic, and prognostic value in CRC. CCNA2 was closely associated with tumor-infiltrating immunocytes, transcription factors, and miRNAs. The knockdown of CCNA2 inhibited the proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), while inducing apoptosis of CRC cells. CCNA2 acted as a target of miR-548x-3p in regulating the biological behavior of CRC cells via the EMT-signaling pathway. CCNA2 is a potential biomarker for the diagnosis, treatment, and prognosis of CRC. The miR-548x-3p-CCNA2 axis plays a pivotal role in regulating the tumorigenesis of CRC through the EMT-signaling pathway. |
| WOS关键词 | CELL-CYCLE ; GENE-EXPRESSION ; WEB SERVER ; PROLIFERATION ; EMT ; TRANSITION ; APOPTOSIS ; INVASION ; SURVIVAL ; TARGETS |
| 资助项目 | Zhejiang Cancer Hospital's Cultivate Foundation of Natural Science Foundation of China[PY2021056] ; Medical Health Science and Technology Project of Zhejiang Province[2020KY081] ; Project of Administration of Traditional Chinese Medicine of Zhejiang Province[2020ZA020] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001591233800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321663]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, Cunchuan; Du, Yian |
| 作者单位 | 1.Zhejiang Univ Technol, Collaborat Innovat Ctr Yangtza River Delta Reg Gre, Hangzhou, Zhejiang, Peoples R China 2.Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 4.Univ Chinese Acad Sci UCAS, Hangzhou Inst Adv Study, Sch Mol Med, Hangzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Hangzhou, Zhejiang, Peoples R China 6.Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Dept Integrated Tradit Chinese & Western Med, Hangzhou, Zhejiang, Peoples R China 7.Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Dept Radiat Phys, Hangzhou, Zhejiang, Peoples R China 8.Univ South China, Affiliated Hosp 1, Dept Pathol, Hengyang, Hunan, Peoples R China 9.Jinan Univ, Affiliated Hosp 1, Res Ctr, Dept Oncol Canc Diag & Therapy, Guangzhou, Guangdong, Peoples R China 10.Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Dept Chest Radiotherapy, Hangzhou, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Xinyi,Qiu, Lanying,Chen, Qiang,et al. Exploring the regulatory mechanism of CCNA2 in colorectal cancer: Insights from multiomics and experimental analysis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2025,301(8):16. |
| APA | Lei, Xinyi.,Qiu, Lanying.,Chen, Qiang.,Liao, Lan.,Yu, Pengfei.,...&Du, Yian.(2025).Exploring the regulatory mechanism of CCNA2 in colorectal cancer: Insights from multiomics and experimental analysis.JOURNAL OF BIOLOGICAL CHEMISTRY,301(8),16. |
| MLA | Lei, Xinyi,et al."Exploring the regulatory mechanism of CCNA2 in colorectal cancer: Insights from multiomics and experimental analysis".JOURNAL OF BIOLOGICAL CHEMISTRY 301.8(2025):16. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。