Uncovering key anti-inflammatory constituents and mechanism of Wuwei Xiaodu Decoction by a combined strategy of phytochemistry, AIDD, network pharmacology, and in vitro and in vivo assay
文献类型：期刊论文
| 作者 | Li, Binghui1,2; Zhou, Zhaoyin1,7; Zhang, Yong1; Zhu, Jing1,6; Liu, Qiyuan1,5; Ma, Jing4; Gong, Zhangbin6; Xu, Zhijian1,5; Shen, Jingshan1,5; Zhu, Weiliang1,5 |
| 刊名 | BMC COMPLEMENTARY MEDICINE AND THERAPIES
 |
| 出版日期 | 2025-09-30 |
| 卷号 | 25期号:1页码:20 |
| 关键词 | Wuwei xiaodu decoction Active components Anti-inflammation effect RAW264.7 cells Mouse model |
| DOI | 10.1186/s12906-025-05072-w |
| 英文摘要 | BackgroundWuwei Xiaodu decoction (WXD), a renowned prescription in traditional Chinese medicine, is widely used to treat various skin diseases, including atopic dermatitis. However, the bioactive constituents and anti-inflammatory mechanisms remain unclear.PurposeThe study was designed to investigate the most important bioactive components and the anti-inflammatory molecular mechanisms of WXD that have not been fully elucidated.Materials and methodsThe mixed herbs of WXD were extracted by different solvents, including ethyl-acetate (EA) and petroleum-ether (PE). HPLC/LC-MS and GC-MS were performed to identify the chemical composition of WXD-EA and WXD-PE, respectively. The RAW264.7 cell line was used to assess the inflammatory responses, while CCK8 assay was employed to assess the cytotoxicity. Nitric oxide (NO) levels were quantified using the Griess assay. The protein expression levels were evaluated by western blot analysis. Nuclear translocation of p-STAT3 were analyzed by immunofluorescence and western blot. The targets and pathways were predicted by open-source databases. 1-chloro-2,4-dinitrobenzene (DNCB)-induced BALB/c mice was utilized for evaluating inflammatory responses. H&E staining was performed to assess the histopathological changes.ResultsThe best anti-inflammatory active fractions of WXD were found to be WXD-PE and WXD-EA.123 compounds from WXD-PE and 7 representative compounds with high content from WXD-EA were successfully identified. The in vitro anti-inflammatory activity studies confirmed that both WXD-PE and WXD-EA can significantly inhibit the production of NO, TNF-alpha, IL-6, and IL-1 beta in LPS-induced RAW264.7 cells, leading to a downregulation of COX-2 and iNOS expression, as well as a reduction in the phosphorylation levels of ERK, p38, and JNK proteins. Notably, the nuclear expression levels of phosphorylated STAT3 were significantly downregulated. Additionally, WXD-H2O and WXD-EA significantly alleviated DNCB-induced acanthosis, lymphocyte and neutrophil infiltration, and collagen over-deposition in the skin of BALB/c mice.ConclusionWXD-PE and WXD-EA, are identified as the most potent anti-inflammatory constituents of WXD as these fractions can effectively inhibit the MAPK signaling pathway and prevent the nuclear translocation of phosphorylated STAT3 in LPS-stimulated RAW264.7 cells. They also showed significant anti-inflammatory activity in the DNCB-induced inflammatory mouse model. |
| WOS关键词 | NITRIC-OXIDE ; MACROPHAGE POLARIZATION ; INFLAMMATION |
| 资助项目 | School of Medicine, Shanghai Jiao Tong University |
| WOS研究方向 | Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001586147500007 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321645]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Zhang, Yong; Shen, Jingshan; Zhu, Weiliang; Chen, Jia |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Henan Univ, Sch Pharm, Kaifeng 475004, Peoples R China 3.Shanghai Jiao Tong Univ, Guizhou Hosp, Shanghai Childrens Med Ctr, Sch Med, Guiyang 550009, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Integrat Med Pediat, Shanghai 200120, Peoples R China 5.Univ Chinese Acad Sci, Sch Pharm, Beijing 100049, Peoples R China 6.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China 7.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Binghui,Zhou, Zhaoyin,Zhang, Yong,et al. Uncovering key anti-inflammatory constituents and mechanism of Wuwei Xiaodu Decoction by a combined strategy of phytochemistry, AIDD, network pharmacology, and in vitro and in vivo assay[J]. BMC COMPLEMENTARY MEDICINE AND THERAPIES,2025,25(1):20. |
| APA | Li, Binghui.,Zhou, Zhaoyin.,Zhang, Yong.,Zhu, Jing.,Liu, Qiyuan.,...&Chen, Jia.(2025).Uncovering key anti-inflammatory constituents and mechanism of Wuwei Xiaodu Decoction by a combined strategy of phytochemistry, AIDD, network pharmacology, and in vitro and in vivo assay.BMC COMPLEMENTARY MEDICINE AND THERAPIES,25(1),20. |
| MLA | Li, Binghui,et al."Uncovering key anti-inflammatory constituents and mechanism of Wuwei Xiaodu Decoction by a combined strategy of phytochemistry, AIDD, network pharmacology, and in vitro and in vivo assay".BMC COMPLEMENTARY MEDICINE AND THERAPIES 25.1(2025):20. |
入库方式： OAI收割
来源：上海药物研究所
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