Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP
文献类型：期刊论文
| 作者 | Ceng FK(曾凡逵)1 ; Liu H(刘红)2; Ma PJ(马鹏军)1,3; Liu G(刘刚)1; Liu G(刘刚)
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| 刊名 | Biotechnology and Bioprocess Engineering
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| 出版日期 | 2013 |
| 卷号 | 18期号:5页码:981-988 |
| 关键词 | potato root water potato protein protease inhibitors expanded bed adsorption Amberlite XAD7HP |
| ISSN号 | 1226-8372 |
| 通讯作者 | 刘刚 |
| 英文摘要 | Potato root water (PRW) contains ~1.5% protein. In this study, expanded bed adsorption (Potato root water (PRW) contains ~1.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ~170 μg/g. The protease inhibitor activity was ~400 mg/g and 11 ~ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW. |
| 学科主题 | 材料与农业工程 |
| 收录类别 | SCI |
| 资助信息 | the earmarked fund for Modern Agro-industry Technology Research System (nycytx-15) and the National Natural Science Foundation of China (31301532) |
| 语种 | 英语 |
| WOS记录号 | WOS:000326040600019 |
| 公开日期 | 2013-12-03 |
| 源URL | [http://210.77.64.217/handle/362003/4347]  |
| 专题 | 兰州化学物理研究所_环境材料与生态化学研究发展中心 |
| 通讯作者 | Liu G(刘刚) |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Res & Dev Ctr Ecomat & Ecochem, Lanzhou 730000, Peoples R China 2.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ceng FK,Liu H,Ma PJ,et al. Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP[J]. Biotechnology and Bioprocess Engineering,2013,18(5):981-988. |
| APA | Ceng FK,Liu H,Ma PJ,Liu G,&刘刚.(2013).Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP.Biotechnology and Bioprocess Engineering,18(5),981-988. |
| MLA | Ceng FK,et al."Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP".Biotechnology and Bioprocess Engineering 18.5(2013):981-988. |
入库方式： OAI收割
来源：兰州化学物理研究所
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