Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish
文献类型：期刊论文
| 作者 | Chen, Jun1,2; Xu, Qiaoqing1,3; Wang, Tiehui1; Collet, Bertrand4; Corripio-Miyar, Yolanda1; Bird, Steve1,5; Xie, Ping2; Nie, Pin2; Secombes, Christopher J.1; Zou, Jun1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 41期号:2页码:137-152 |
| 关键词 | Fish Teleosts Vertebrates CXC chemokines |
| ISSN号 | 0145-305X |
| 通讯作者 | Secombes, CJ (reprint author), Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland. |
| 中文摘要 | In this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay. (C) 2013 Elsevier Ltd. All rights reserved. |
| 英文摘要 | In this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; PRO-INFLAMMATORY CYTOKINES ; RAINBOW-TROUT ; MOLECULAR CHARACTERIZATION ; EDWARDSIELLA-ICTALURI ; EXPRESSION ANALYSIS ; CC CHEMOKINES ; IFN-GAMMA ; IDENTIFICATION ; INTERLEUKIN-8 |
| 收录类别 | SCI |
| 资助信息 | Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] |
| 语种 | 英语 |
| WOS记录号 | WOS:000323870800005 |
| 公开日期 | 2014-01-03 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19569]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Yangtze Univ, Sch Anim Sci, Jinzhou 434020, Hubei Province, Peoples R China 4.Marine Scotland Sci, Aberdeen AB11 9DB, Scotland 5.Univ Waikato, Sch Biol Sci, Dept Sci & Engn, Hamilton 3240, New Zealand |
| 推荐引用方式 GB/T 7714 | Chen, Jun,Xu, Qiaoqing,Wang, Tiehui,et al. Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(2):137-152. |
| APA | Chen, Jun.,Xu, Qiaoqing.,Wang, Tiehui.,Collet, Bertrand.,Corripio-Miyar, Yolanda.,...&Zou, Jun.(2013).Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(2),137-152. |
| MLA | Chen, Jun,et al."Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.2(2013):137-152. |
入库方式： OAI收割
来源：水生生物研究所
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