Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection
文献类型：期刊论文
| 作者 | Wang, Chih-Hung; Lee, You-Hsun; Kuo, Hsin-Tzu; Liang WF(梁文峰); Li WJ(李文荣) ; Lee, Gwo-Bin
|
| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2014 |
| 卷号 | 14期号:3页码:592-601 |
| ISSN号 | 1473-0197 |
| 产权排序 | 3 |
| 通讯作者 | Lee, Gwo-Bin |
| 中文摘要 | Gene transfection is an important technology for various biological applications. The exogenous DNA is commonly delivered into cells by using a strong electrical field to form transient pores in cellular membranes. However, the high voltage required in this electroporation process may cause cell damage. In this study, a dielectrophoretically-assisted electroporation was developed by using light-activated virtual microelectrodes in a new microfluidic platform. The DNA electrotransfection used a low applied voltage and an alternating current to enable electroporation and transfection. Single or triple fluorescencecarrying plasmids were effectively transfected into various types of mammalian cells, and the fluorescent proteins were successfully expressed in live transfected cells. Moreover, the multi-triangle optical pattern that was projected onto a photoconductive layer to generate localized non-uniform virtual electric fields was found to have high transfection efficiency. The developed dielectrophoretically-assisted electroporation platform may provide a simpler system for gene transfection and could be widely applied in many biotechnological fields. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | CELL-LYSIS DEVICE ; GENE DELIVERY ; ELECTRIC-FIELD ; TISSUE ; SEPARATION ; SYSTEM ; MICRO |
| 收录类别 | SCI |
| 资助信息 | National Science Council in Taiwan [NSC102-2218-E-007-001]; Towards a World-Class University |
| 语种 | 英语 |
| WOS记录号 | WOS:000328911400018 |
| 源URL | [http://ir.sia.cn/handle/173321/14716]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Wang, Chih-Hung,Lee, You-Hsun,Kuo, Hsin-Tzu,et al. Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection[J]. LAB ON A CHIP,2014,14(3):592-601. |
| APA | Wang, Chih-Hung,Lee, You-Hsun,Kuo, Hsin-Tzu,Liang WF,Li WJ,&Lee, Gwo-Bin.(2014).Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection.LAB ON A CHIP,14(3),592-601. |
| MLA | Wang, Chih-Hung,et al."Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection".LAB ON A CHIP 14.3(2014):592-601. |
入库方式： OAI收割
来源：沈阳自动化研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。