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Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics
文献类型：期刊论文
| 作者 | Liang WF(梁文峰); Zhao YL(赵玉良); Liu LQ(刘连庆) ; Wang YC(王越超); Dong ZL(董再励); Li WJ(李文荣); Li GB(李国宾); Xiao XB(肖秀斌); Zhang WJ(张伟京)
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:3页码:1-9 |
| 关键词 | DIELECTROPHORETIC ODEP FORCE MECHANICAL-PROPERTIES ELECTRIC-FIELD CANCER-CELLS MANIPULATION MICROSCOPY ENRICHMENT SYSTEM DEVICE IMAGES |
| ISSN号 | 1932-6203 |
| 产权排序 | 1 |
| 通讯作者 | 刘连庆 ; 李文荣 |
| 中文摘要 | Early stage detection of lymphoma cells is invaluable for providing reliable prognosis to patients. However, the purity of lymphoma cells in extracted samples from human patients' marrow is typically low. To address this issue, we report here our work on using optically-induced dielectrophoresis (ODEP) force to rapidly purify Raji cells' (a type of Burkitt's lymphoma cell) sample from red blood cells (RBCs) with a label-free process. This method utilizes dynamically moving virtual electrodes to induce negative ODEP force of varying magnitudes on the Raji cells and RBCs in an optically-induced electrokinetics (OEK) chip. Polarization models for the two types of cells that reflect their discriminate electrical properties were established. Then, the cells' differential velocities caused by a specific ODEP force field were obtained by a finite element simulation model, thereby established the theoretical basis that the two types of cells could be separated using an ODEP force field. To ensure that the ODEP force dominated the separation process, a comparison of the ODEP force with other significant electrokinetics forces was conducted using numerical results. Furthermore, the performance of the ODEP-based approach for separating Raji cells from RBCs was experimentally investigated. The results showed that these two types of cells, with different concentration ratios, could be separated rapidly using externally-applied electrical field at a driven frequency of 50 kHz at 20 V-pp. In addition, we have found that in order to facilitate ODEP-based cell separation, Raji cells' adhesion to the OEK chip's substrate should be minimized. This paper also presents our experimental results of finding the appropriate bovine serum albumin concentration in an isotonic solution to reduce cell adhesion, while maintaining suitable medium conductivity for electrokinetics-based cell separation. In short, we have demonstrated that OEK technology could be a promising tool for efficient and effective purification of Raji cells from RBCs. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | DIELECTROPHORETIC ODEP FORCE ; MECHANICAL-PROPERTIES ; ELECTRIC-FIELD ; CANCER-CELLS ; MANIPULATION ; MICROSCOPY ; ENRICHMENT ; SYSTEM ; DEVICE ; IMAGES |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [61107043]; CAS-Croucher Joint Lab Scheme [9500011] |
| 语种 | 英语 |
| WOS记录号 | WOS:000332485800056 |
| 源URL | [http://ir.sia.cn/handle/173321/14753]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Liang WF,Zhao YL,Liu LQ,et al. Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics[J]. PLOS ONE,2014,9(3):1-9. |
| APA | Liang WF.,Zhao YL.,Liu LQ.,Wang YC.,Dong ZL.,...&Zhang WJ.(2014).Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics.PLOS ONE,9(3),1-9. |
| MLA | Liang WF,et al."Rapid and Label-Free Separation of Burkitt's Lymphoma Cells from Red Blood Cells by Optically-Induced Electrokinetics".PLOS ONE 9.3(2014):1-9. |
入库方式： OAI收割
来源：沈阳自动化研究所
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