Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia
文献类型：期刊论文
| 作者 | Geng, Xin1,2; Feng, Jianbin1,2; Liu, Shikai1,2; Wang, Yaping1,2,3; Arias, Covadonga1,2; Liu, Zhanjiang1,2 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 169页码:38-50 |
| 关键词 | Fish Gene Hypoxia HIF FIH-1 |
| ISSN号 | 1096-4959 |
| 通讯作者 | Liu, ZJ (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA. |
| 中文摘要 | Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved. |
| 英文摘要 | Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIP-1 alpha, HIF-2 alpha a, HIF-2 alpha b, HIF-3 alpha, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-alpha genes at pre-translational levels. In most tissues, the HIF-alpha mRNAs were down-regulated 15 h but up-regulated 5 h after hypoxia treatment. Of these HIP-alpha mRNAs, the expression of HIF-3 alpha mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1 alpha and HIF-2 alpha genes, suggesting its importance in responses to hypoxia. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | TUMOR-SUPPRESSOR PROTEIN ; EELPOUT ZOARCES-VIVIPARUS ; MESSENGER-RNA EXPRESSION ; INDUCED GENE-EXPRESSION ; HIPPEL-LINDAU PROTEIN ; REAL-TIME PCR ; OXIDATIVE STRESS ; FACTOR 1-ALPHA ; PROLINE HYDROXYLATION ; PROLYL HYDROXYLATION |
| 收录类别 | SCI |
| 资助信息 | Agriculture and Food Research Initiative Competitive Grant Program of the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; China Scholarship Council (CSC) |
| 语种 | 英语 |
| WOS记录号 | WOS:000331485100006 |
| 公开日期 | 2014-08-08 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20047]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA 2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Xin,Feng, Jianbin,Liu, Shikai,et al. Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,169:38-50. |
| APA | Geng, Xin,Feng, Jianbin,Liu, Shikai,Wang, Yaping,Arias, Covadonga,&Liu, Zhanjiang.(2014).Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,169,38-50. |
| MLA | Geng, Xin,et al."Transcriptional regulation of hypoxia inducible factors alpha (HIF-alpha) and their inhibiting factor (FIH-1) of channel catfish (Ictalurus punctatus) under hypoxia".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 169(2014):38-50. |
入库方式： OAI收割
来源：水生生物研究所
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