A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers
文献类型：期刊论文
| 作者 | Gu, Dongyu; Yang, Yi; Bakri, Mahinur; Chen, Qibin; Xin, Xuelei ; Aisa, Haji Akber
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2013 |
| 卷号 | 24期号:6页码:661-670 |
| 关键词 | LC/QTOF-MS/MS flavonoids protein tyrosine phosphatase 1B inhibition tannins Rosa rugosa Thunb flowers |
| ISSN号 | 0958-0344 |
| 通讯作者 | Aisa, HA |
| 中文摘要 | IntroductionRosa rugosa flowers used as herbal medicine possess many activities. A fraction extracted by ethyl acetate exhibited strong inhibitive activity against protein tyrosine phosphatase 1B (PTP1B) in vitro. ObjectiveEstablish an efficient method of LC coupled to quadrupole time-of-flight (QTOF) with tandem MS/MS to investigate the compositions in the active fraction. MethodsChemical compositions were separated and investigated by LC/QTOF-MS/MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. The maximal structural information was obtained for the identification of components. ResultsA total of 75 compounds including tannins, their related compounds and flavonoids were identified or partially characterised according to accurate mass and the characteristic fragments at low and high CE. Meanwhile, the fragmentation pathways of gallotannins and ellagitannins (hexahydroxydiphenoyl group and lactonised valoneoyl group) were studied and proposed and were used to trace tannins in crude extracts. ConclusionThe results suggest that this fraction is a source of PTP1B inhibitory activity with a potential for treating diabetes. Copyright (c) 2013 John Wiley & Sons, Ltd. As a traditional herbal medicine, Rosa rugosa flowers possess many activities. In this study, a LC-MS method was established for investigation of chemical compositions in a fraction with the PTP 1B inhibitory activity from Rosa rugosa. And 75 chemical compositions in this fraction were identified or tentatively characterized. The fragmentation pathways of gallotannins and ellagitannins were proposed. Knowing the phytochemical profiles of PTP 1B inhibitory activity fraction of this plant gives insights to its potential application to promote health. |
| 收录类别 | SCI |
| 资助信息 | China National Science Fund for Distinguished Young Scholars CAS/SAFEA International Partnership Program for Creative Research Teams and Major State Basic Research Development Program |
| 公开日期 | 2014-11-11 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/3858]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Dongyu,Yang, Yi,Bakri, Mahinur,et al. A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):661-670. |
| APA | Gu, Dongyu,Yang, Yi,Bakri, Mahinur,Chen, Qibin,Xin, Xuelei,&Aisa, Haji Akber.(2013).A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.PHYTOCHEMICAL ANALYSIS,24(6),661-670. |
| MLA | Gu, Dongyu,et al."A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers".PHYTOCHEMICAL ANALYSIS 24.6(2013):661-670. |
入库方式： OAI收割
来源：新疆理化技术研究所
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