Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption
文献类型：期刊论文
| 作者 | Xia, Tingting1; Guan, Yueping1; Yang, Mingzhu1; Xiong, Wubin1; Wang, Ning2; Zhao, Shen1; Guo, Chen2 |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2014-02-20 |
| 卷号 | 443期号:FEB.页码:552-559 |
| 关键词 | Fe3O4-PEI NPs Protein adsorption BHb BSA Adsorption capacity Selectivity |
| ISSN号 | 0927-7757 |
| 其他题名 | Colloid Surf. A-Physicochem. Eng. Asp. |
| 中文摘要 | We report the modification of amine-functioned Fe3O4 nanoparticles (provided by the Laboratory of BioMedical Materials of University of Science and Technology Beijing) with polyethylenimine (PEI) using glutaraldehyde (GA) as a linker to increase the concentration of primary amine groups on the surface. The resultant Fe3O4-PEI NPs with immobilized iminodiacetic acid (IDA)-Cu2+ groups were applied to investigate the adsorption capacity and selectivity of model proteins: bovine hemoglobin (BHb) and bovine serum albumin (BSA). The Fe3O4-PEI NPs were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), particle analyzer, attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), ninhydrin colorimetry and thermogravimetric analysis (TGA). The results showed that the well dispersed Fe3O4-PEI NPs had an average size of 172.6 nm and were superparamagnetic with saturation magnetization of 71.11 emu/g. The characteristic peaks of PEI appeared in the ATR-FTIR spectra of Fe3O4-PEI NPs. The study of zeta potential measurement presented that the isoelectric points of the Fe3O4-PEI NPs increased to 10.5. Ninhydrin colorimetry analysis of primary amine groups showed that the concentration of primary amine groups on the surface of Fe3O4-PEI NPs increased to 0.45 mmol/g and that of TGA is 0.53 mmol/g. These results showed that PEI had been successfully modified on the surface of amine-functioned Fe3O4 NPs and the concentration of primary amine groups was significantly increased. The analysis of protein adsorption indicated that the adsorption capacity of BHb was up to about 6000 mg/g and that of BSA was only about 2000 mg/g, which was about one third of that of BHb. The research on the selectivity of protein adsorption was conducted by HPLC. The result showed that the adsorption capacity of BHb was 2.5 times as high as that of BSA, which further proved Fe3O4-PEI-Cu2+ NPs shows specific adsorption for proteins. These properties suggested the potential of the Fe3O4-PEI-Cu2+ NPs as protein adsorbent. (C) 2013 Elsevier B.V. All rights reserved. |
| 英文摘要 | We report the modification of amine-functioned Fe3O4 nanoparticles (provided by the Laboratory of BioMedical Materials of University of Science and Technology Beijing) with polyethylenimine (PEI) using glutaraldehyde (GA) as a linker to increase the concentration of primary amine groups on the surface. The resultant Fe3O4-PEI NPs with immobilized iminodiacetic acid (IDA)-Cu2+ groups were applied to investigate the adsorption capacity and selectivity of model proteins: bovine hemoglobin (BHb) and bovine serum albumin (BSA). The Fe3O4-PEI NPs were characterized by transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), particle analyzer, attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), ninhydrin colorimetry and thermogravimetric analysis (TGA). The results showed that the well dispersed Fe3O4-PEI NPs had an average size of 172.6 nm and were superparamagnetic with saturation magnetization of 71.11 emu/g. The characteristic peaks of PEI appeared in the ATR-FTIR spectra of Fe3O4-PEI NPs. The study of zeta potential measurement presented that the isoelectric points of the Fe3O4-PEI NPs increased to 10.5. Ninhydrin colorimetry analysis of primary amine groups showed that the concentration of primary amine groups on the surface of Fe3O4-PEI NPs increased to 0.45 mmol/g and that of TGA is 0.53 mmol/g. These results showed that PEI had been successfully modified on the surface of amine-functioned Fe3O4 NPs and the concentration of primary amine groups was significantly increased. The analysis of protein adsorption indicated that the adsorption capacity of BHb was up to about 6000 mg/g and that of BSA was only about 2000 mg/g, which was about one third of that of BHb. The research on the selectivity of protein adsorption was conducted by HPLC. The result showed that the adsorption capacity of BHb was 2.5 times as high as that of BSA, which further proved Fe3O4-PEI-Cu2+ NPs shows specific adsorption for proteins. These properties suggested the potential of the Fe3O4-PEI-Cu2+ NPs as protein adsorbent. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | COMPOSITE NANOPARTICLES ; AFFINITY LIGANDS ; PURIFICATION ; SEPARATION ; POLYMERIZATION ; MICROSPHERES ; SUPPORTS ; ENZYMES ; BEADS ; ACID |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000342501300076 |
| 公开日期 | 2014-11-02 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11660]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Tingting,Guan, Yueping,Yang, Mingzhu,et al. Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2014,443(FEB.):552-559. |
| APA | Xia, Tingting.,Guan, Yueping.,Yang, Mingzhu.,Xiong, Wubin.,Wang, Ning.,...&Guo, Chen.(2014).Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,443(FEB.),552-559. |
| MLA | Xia, Tingting,et al."Synthesis of polyethylenimine modified Fe3O4 nanoparticles immobilized Cu2+ for highly efficient proteins adsorption".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 443.FEB.(2014):552-559. |
入库方式： OAI收割
来源：过程工程研究所
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