Spectrum of LKB1, EGFR, and KRAS Mutations in Chinese Lung Adenocarcinomas
文献类型：期刊论文
| 作者 | Gao, B; Sun, YH; Zhang, JH; Ren, Y; Fang, R; Han, XK; Shen, L; Liu, XY; Pao, W; Chen, HQ |
| 刊名 | JOURNAL OF THORACIC ONCOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:8页码:1130-1135 |
| 关键词 | Chinese lung adenocarcinoma LKB1 EGFR KRAS Mutation |
| 通讯作者 | Ji, HB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,hqchen1@yahoo.com |
| 英文摘要 | Introduction: Somatic LKB1 mutations are found in lung adenocarcinomas at different frequencies in Caucasian and East Asian (Japanese and Korean) populations. This study was designed to characterize the frequency of LKB1 mutations, their relationship to EGFR and KRAS mutations, and their associated clinicopathologic characteristics in Chinese patients. Methods: Two hundred thirty-nine lung adenocarcinomas consecutively collected from October 2007 to July 2009 were dissected into 3 to 4 small (3 mm) pieces for histopathological analyses of tumor content. Genomic DNA and/or cDNA from 86 samples with more than 70% tumor content were used for sequencing of LKB1 (exons 1-9), EGFR (exons 18-21), and KRAS (exon 2). LKB1 germline mutation status was determined by sequencing of genomic DNA from matched histologically distant lung tissues that are histologically normal. Results: 6.9% of lung adenocarcinomas harbored LKB1 somatic mutations. A total of 10.5% of patients had an LKB1 germline polymorphism, F354L. Interestingly, in two of these patients, tumors displayed loss of heterozygosity at this allele. EGFR kinase domain and KRAS mutations were found in 66.3% and 2.3% of Chinese lung adenocarcinomas, respectively. Concurrent LKB1 and EGFR somatic mutations were observed in one patient. Both KRAS-mutant tumors harbored LKB1 mutations. Conclusions: These data provide important clinical and molecular characteristics of lung adenocarcinomas from Chinese patients. |
| 学科主题 | Oncology ; Respiratory System |
| 类目[WOS] | Oncology ; Respiratory System |
| 关键词[WOS] | PEUTZ-JEGHERS-SYNDROME ; GROWTH-FACTOR-RECEPTOR ; SOMATIC MUTATIONS ; CANCER PATIENTS ; GENE-MUTATIONS ; CLINICOPATHOLOGICAL FEATURES ; TUMOR-SUPPRESSOR ; NEVER SMOKERS ; GEFITINIB ; INHIBITORS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280366700005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1037]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, B,Sun, YH,Zhang, JH,et al. Spectrum of LKB1, EGFR, and KRAS Mutations in Chinese Lung Adenocarcinomas[J]. JOURNAL OF THORACIC ONCOLOGY,2010,5(8):1130-1135. |
| APA | Gao, B.,Sun, YH.,Zhang, JH.,Ren, Y.,Fang, R.,...&Ji, HB.(2010).Spectrum of LKB1, EGFR, and KRAS Mutations in Chinese Lung Adenocarcinomas.JOURNAL OF THORACIC ONCOLOGY,5(8),1130-1135. |
| MLA | Gao, B,et al."Spectrum of LKB1, EGFR, and KRAS Mutations in Chinese Lung Adenocarcinomas".JOURNAL OF THORACIC ONCOLOGY 5.8(2010):1130-1135. |
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