Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody
文献类型：期刊论文
| 作者 | Lin, Y; Liu, ZD; Jiang, JM; Jiang, ZQ; Ji, YY; Sun, B |
| 刊名 | CELLULAR & MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 1期号:2页码:137-141 |
| 关键词 | EGFR prokaryotic expression monoclonal antibody intracellular domain |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Immunol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | To prepare monoclonal antibody specific to epidermal growth factor receptor (EGFR) intracellular domain, its gene was amplified from total RNA of A431 cell by RT-PCR. Then the gene was cloned into prokaryotic vector pET30a(+). The recombinant plasmid was transformed into E. coli BL21 (DE3) strain for protein expression. Recombinant protein was induced with IPTG and purified using Ni2+-NTA agarose. Then the anti-EGFR monoclonal antibody (mAb) was prepared with classical hybridoma technique. Positive clones were selected using indirect enzyme-linked immunoabsorbent assay (ELISA). Totally 4 hybridoma clones were obtained and these mAbs were IgG1 (3 clones) and IgG2a (1 clone), respectively. Their light chains were all kappa chains. Western blotting analysis and confocal immunofluorescence assays demonstrated that mAbs could specifically recognize EGFR expressing on A431 carcinoma cell line. The mAbs will be useful in the study of EGFR-mediated signal transduction. |
| 学科主题 | Immunology |
| 类目[WOS] | Immunology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000208922200009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2034]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lin, Y,Liu, ZD,Jiang, JM,et al. Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2004,1(2):137-141. |
| APA | Lin, Y,Liu, ZD,Jiang, JM,Jiang, ZQ,Ji, YY,&Sun, B.(2004).Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody.CELLULAR & MOLECULAR IMMUNOLOGY,1(2),137-141. |
| MLA | Lin, Y,et al."Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody".CELLULAR & MOLECULAR IMMUNOLOGY 1.2(2004):137-141. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。