Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope
文献类型：期刊论文
| 作者 | Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励) ; Guan, Yifu
|
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5页码:1-7 |
| ISSN号 | 2045-2322 |
| 产权排序 | 2 |
| 通讯作者 | Guan, Yifu |
| 中文摘要 | As one of the most crucial properties of DNA, the structural stability and the mechanical strength are attracting a great attention. Here, we take advantage of high force resolution and high special resolution of Atom Force Microscope and investigate the mechanical force of DNA duplexes. To evaluate the base pair hydrogen bond strength and base stacking force in DNA strands, we designed two modes (unzipping and stretching) for the measurement rupture forces. Employing k-means clustering algorithm, the ruptured force are clustered and the mean values are estimated. We assessed the influence of experimental parameters and performed the force evaluation for DNA duplexes of pure dG/dC and dA/dT base pairs. The base binding strength of single dG/dC and single dA/dT were estimated to be 20.0 +/- 0.2 pN and 14.0 +/- 0.3 pN, respectively, and the base stacking interaction was estimated to be 2.0 +/- 0.1 pN. Our results provide valuable information about the quantitative evaluation of the mechanical properties of the DNA duplexes. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SINGLE-MOLECULE ; COMPLEMENTARY STRANDS ; OPTICAL TWEEZERS ; MAGNETIC BEADS ; DOUBLE HELIX ; STABILITY ; SPECTROSCOPY ; OLIGONUCLEOTIDE ; ELASTICITY ; RESOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351181000007 |
| 源URL | [http://ir.sia.ac.cn/handle/173321/16226]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 推荐引用方式 GB/T 7714 | Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope[J]. SCIENTIFIC REPORTS,2015,5:1-7. |
| APA | Zhang, Tianbiao,Zhang CL,Dong ZL,&Guan, Yifu.(2015).Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope.SCIENTIFIC REPORTS,5,1-7. |
| MLA | Zhang, Tianbiao,et al."Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope".SCIENTIFIC REPORTS 5(2015):1-7. |
入库方式： OAI收割
来源：沈阳自动化研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。