中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
机构
采集方式
内容类型
发表日期
学科主题
筛选

浏览/检索结果: 共8条,第1-8条 帮助

条数/页: 排序方式:
基于微波散射实验的油种识别研究 期刊论文  OAI收割
海洋与湖沼, 2023, 卷号: 54, 期号: 1, 页码: 30-43
作者:  
马靖;  过杰
  |  收藏  |  浏览/下载:11/0  |  提交时间:2024/10/28
基于微波散射实验的油种识别研究 期刊论文  OAI收割
海洋与湖沼, 2023, 卷号: 54, 期号: 1, 页码: 30-43
作者:  
马靖;  过杰
  |  收藏  |  浏览/下载:32/0  |  提交时间:2023/08/16
烟台牟平海洋牧场季节性低氧对大型底栖动物群落的生态效应 期刊论文  OAI收割
生物多样性, 2019, 卷号: 27, 期号: 2, 页码: 200-210
作者:  
李宝泉
  |  收藏  |  浏览/下载:26/0  |  提交时间:2020/06/17
低氧  群落结构  群落恢复  敏感种  机会种  hypoxia  community structure  community recovery  sensitive species  opportunistic species  Hypoxia is a common phenomenon in the world's oceans, especially in the shallow waters of coastal zones. Rates of hypoxia are increasing due to global climatic changes and anthropogenic activities. Hypoxia can cause mass mortality of marine animals and can have severe negative impacts on marine ecosystems. To better understand the effects of hypoxia on macrobenthic communities, a survey was carried out in the Muping Marine Ranch (Yantai) during June, August and September of 2016. Results showed that seasonal hypoxia led to changes in benthic community structure, especially in terms of species composition and dominant species. The dominant species were Polychaeta Lumbrinereis latreilli, Sternaspis scutata and Mollusca Endopleura lubrica in summer. The opportunistic species Lumbrinereis latreilli increased, whereas, sensitive species such as Leptomya minuta, Glycera chirori, Upogebia major, Pontocrates altamarimus, Eriopisella sechellensis decreased during the hypoxic period of August. Hypoxia also reduced biodiversity indices. The effect of hypoxia on abundance and biomass were not significant, mainly because of the increase in the opportunistic Lumbrinereis latreilli, which counteracted the decline in abundance and biomass of other species. Individual physiological tolerance to hypoxia was different among species. Lumbrinereis latreilli showed higher tolerance to hypoxia in dissolved oxygen (DO) = 1.0 mg/L compared to other species. Some sensitive species, such as Leptomya minuta, Upogebia major, Pontocrates altamarimus and Eriopisella sechellensis showed lower tolerance when DO < 2.5 mg/L. When DO increased to 2.5 mg/L, the macrobenthic community start to recover gradually. The recovery extent and time needed were closely related to the degree of seasonal hypoxia.  
黄土丘陵区撂荒群落演替序列种根系对氮素施肥方式 和水平的形态响应 期刊论文  OAI收割
生态学报, 2017, 卷号: 37, 期号: 9, 页码: 2913-2925
作者:  
高艳;  王月;  李伟伟;  隋媛媛;  杜峰
  |  收藏  |  浏览/下载:33/0  |  提交时间:2019/04/26
脉冲管制冷机第三种直流敏感性实验研究 会议论文  OAI收割
中国工程热物理学会, 2012
顾超; 周远; 王俊杰; 朱文秀; 季伟
收藏  |  浏览/下载:26/0  |  提交时间:2013/03/20
一种管道土壤腐蚀传感器 专利  OAI收割
专利类型: 实用新型, 专利号: CN201020240991.8, 申请日期: 2011-01-05, 公开日期: 2011-01-05
杨锦林; 黄彦良; 郑凤; 李言涛; 张仕强; 侯保荣; 王雨生
收藏  |  浏览/下载:18/0  |  提交时间:2014/08/04
电阻磁流体力学模拟的CESE方法 期刊论文  OAI收割
空间科学学报, 2010, 卷号: 30, 期号: 3, 页码: 211-220
刘元昕; 纪珍; 冯学尚; 周玉芬
收藏  |  浏览/下载:60/0  |  提交时间:2014/04/30
栉孔扇贝G-型溶菌酶基因多态性标记筛选及其辅助育种方法 专利  OAI收割
专利类型: 发明, 专利号: CN200810015048.4, 申请日期: 2008-09-03, 公开日期: 2008-09-03
宋林生; 李凌; 赵建民
收藏  |  浏览/下载:37/0  |  提交时间:2014/08/04
1  2)抗病群体和敏感群体的制备  3)抗病相关G-型溶菌酶基因标记的筛选  4)携带抗病相关基因标记个体的快速筛查。1)栉孔扇贝G-型溶菌酶基因启动区部分序列的克隆:参照分子克隆所述方法从栉孔扇贝闭壳肌中提取总DNA  根据已知的G-型溶菌酶基因序列在其启动子区设计引物  即从不同海区和养殖场收集栉孔扇贝个体  PCR扩增42个敏感个体和40个抗病个体的G-型溶菌酶启动区序列后  其中-754ID和-754II个体在抗病群体和敏感群体中出现的频率无显著差别  一种栉孔扇贝抗病相关G-型溶菌酶基因标记  克隆得到一段762个碱基的DNA序列  用病原菌浸泡感染  针对-754TATCTCGATCAGG插入/缺失(I/D)及-391A/G转换分别选用Taq I和Hap II对PCR产物进行酶切  而-391AA个体在敏感群体中出现的频率显著高于抗病群体  其特征在于它的技术内容包括以下四个方面:1)栉孔扇贝G-型溶菌酶基因启动区部分序列的克隆  连入T载体后进行测序  根据死亡时间判断扇贝对病原菌感染抵抗能力  聚丙烯酰胺凝胶电泳后  -391AG个体在抗病群体中出现的频率显著高于敏感群体。因此  获得其核苷酸序列。2)抗病群体和敏感群体的制备:采用人工感染的方法获得抗病群体和敏感群体  将扇贝分为抗病群体和敏感群体。3)抗病相关G-型溶菌酶基因标记的筛选:对来自5个个体的10个克隆进行了测序  各发现两种基因型  将-391AA作为疾病敏感相关的G-型溶菌酶基因标记  发现了10处多态性位点  -754II  而将-391AG作为抗病相关的G-型溶菌酶基因标记。4)携带抗病相关基因标记个体的快速筛查:取栉孔扇贝个体全血1μl作为模版  -754ID  PCR扩增G-型溶菌酶基因启动区序列  -391AA和-391AG  用Hap II对产物进行酶切  聚丙烯酰胺凝胶电泳分型后  选择-391AG个体作为抗病个体。