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	Influence of sensitizer concentration on the upconversion luminescence of NaYF4: Yb3+, Er3+ nanoparticles (EI CONFERENCE) 会议论文 OAI收割 2012 2nd International Conference on Machinery, Materials Science and Engineering Applications, MMSE 2012, June 16, 2012 - June 17, 2012, Wuhan, China 作者: Zhao J.; Zhao Y.; Zhao Y.; Zhao Y. 收藏 \| 浏览/下载：24/0 \| 提交时间：2013/03/25 A series of NaYF4: Yb3+ Er3+ nanoparticles with different doping concentration were successfully prepared by the combination of coprecipitation and hydrothermal methods using sodium citrate as chelator. Upconversion luminescence can be observed in all of the samples at room temperature. Fixed the Er3+ concentration with 1 mol% unchanged the effect of the Yb3+ concentration on the upconversion luminescence of NaYF4: Yb3+ Er3+ samples were studied when the doped Yb3+ concentrations were 0 mol% 1 mol% 2 mol% 5 mol% 10 mol% and 20 mol% respectively. As increasing the Yb 3+ concentration the total intensity of the upconversion luminescence of the samples is increasing while the ratio of red to green emission increases firstly and then decreases. The maximum ratio value is 22.1 when the Yb3+ concentration is 10 mol%. It is found that the cross relaxation and cooperative sensitization effect are the reasons for the phenomenon above. (2012) Trans Tech Publications.
	A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE) 会议论文 OAI收割 International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009, Beijing, China 作者: Song K.-F.; Li J.-L.; Zhang W.-L.; Wang Y.-L. 收藏 \| 浏览/下载：37/0 \| 提交时间：2013/03/25 In the device a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least the sample fluorescence through the integrating sphere and come to the grating monochromator The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process signal-to-noise ratio(SNR) will be improved also adjust delay time ampling frequency and sampling number arbitrarily. By testing the sensitivity is 10-12mol/L(substance marked by Eu3+) examination repeat is &le2.5% examination linearity is from 10 -8mol/L to 10-12mol/L correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody and solve the tumor genetic variation the virus protein detection. 2009 SPIE.