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CAS IR Grid
机构
上海应用物理研究所 [2]
生物物理研究所 [1]
长春光学精密机械与物... [1]
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OAI收割 [4]
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期刊论文 [3]
会议论文 [1]
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2016 [1]
2014 [1]
2010 [1]
2009 [1]
学科主题
Biochemist... [1]
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Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
期刊论文
OAI收割
SCIENCE CHINA-CHEMISTRY, 2016, 卷号: 59, 期号: 11, 页码: 1519-1524
作者:
Wang, SP
;
Deng, SH
;
Cai, XQ
;
Hou, SG
;
Li, JJ
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浏览/下载:50/0
  |  
提交时间:2017/03/02
telomere
superresolution imaging
stimulated emission depletion (STED) microscopy
fluorescence in situ hybridization (FISH)
Nanoprobes for super-resolution fluorescence imaging at the nanoscale
期刊论文
OAI收割
SCIENCE CHINA-CHEMISTRY, 2014, 卷号: 57, 期号: 1, 页码: 100—106
Hou, SG
;
Liang, L
;
Deng, SH
;
Chen, JF
;
Huang, Q
;
Cheng, Y
;
Fan, CH
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  |  
浏览/下载:25/0
  |  
提交时间:2015/03/13
RESONANCE ENERGY-TRANSFER
DIFFRACTION RESOLUTION LIMIT
FIELD OPTICAL NANOSCOPY
GROUND-STATE-DEPLETION
CDSE/ZNS QUANTUM DOTS
STED MICROSCOPY
LOCALIZATION MICROSCOPY
STIMULATED-EMISSION
SCALE RESOLUTION
LIVING CELLS
Mechanism of STED microscopy and analysis of the factors affecting resolution (EI CONFERENCE)
会议论文
OAI收割
6th International Symposium on Precision Engineering Measurements and Instrumentation, August 8, 2010 - August 11, 2010, Hangzhou, China
Yang P.
;
Ai H.
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浏览/下载:22/0
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提交时间:2013/03/25
Stimulated emission depletion (STED) microscopy exploits nonlinear saturable optical transition of fluorescent molecules
allowed to overcome Abbe's diffraction-limit and provides diffraction-unlimited resolution in far-field optical microscopy. We elaborate the mechanism of STED and the conditions of depletion. The formula of STED microcopy resolution is deduced through effective point spread function (E-PSF). The STED system resolution is mainly dominated by the quality of the fluorescence depletion patterns in the focal plane. The depletion pattern is mainly affected by STED beam intensity
polarization
phase plate
primary aberrations
STED pulse shape
pulse duration and delay time. In this paper
we found related models and simulate the relationship between the depletion patterns and the parameters
and put forward effective approach to enhance the system resolution. 2010 SPIE.
Introduction to Theories of Several Super-resolution Fluorescence Microscopy Methods and Recent Advance in The Field
期刊论文
OAI收割
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2009, 卷号: 36, 期号: 12, 页码: 1626-1634
Lue ZhiJian
;
lu jingze
;
陆敬泽
;
Wu YaQiong
;
Chen Liangyi
;
陈良怡
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浏览/下载:102/0
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提交时间:2013/12/25
super-resolution fluorescence microscopy
point spread function (PSF)
photoactivated localization microscopy (PALM)
stochastic optical reconstruction microscopy (STORM)
stimulated emission depletion (STED)
saturated structure illumination microscopy (SSIM)