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近海环境中抗生素分析样品前处理技术的研究进展 期刊论文 OAI收割
色谱, 2020, 卷号: 38, 期号: 1, 页码: 95-103
作者: 吕敏; 陈令新

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抗生素近海前处理技术综述 antibiotics coastal pretreatment techniques review The widespread and massive usage of antibiotics for the prevention and treatment of diseases in human and animals have resulted in their continuous discharge into the environment, and finally, into the coastal environment through various pathways. Accumulation of antibiotics in aquatic organisms poses a great threat to ecological and human health, especially because it facilitates the generation and spread of bacterial resistance. Given the diverse range of antibiotics with various features, as well as their presence at low concentrations in the environment, it is imperative to develop pretreatment techniques for antibiotics in various matrices. In this paper, the pretreatment methods proposed in recent decades for antibiotics in coastal water, sediment, and biota are summarized, with emphasis on commonly used methods such as solid-phase extraction, solid-liquid extraction, matrix solid-phase dispersion, and QuEChERS. Additionally, the potential factors influencing the extraction performance and clean-up efficiency are analyzed. The advantages and disadvantages of the methods and their development are finally discussed.

碳氮稳定同位素比值在潮间带大型底栖动物组织间差异性研究 期刊论文 OAI收割
海洋学报, 2019, 卷号: 41, 期号: 4, 页码: 74-82
作者: 李宝泉

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潮间带大型底栖动物碳氮稳定同位素食源和营养级分析采样部位选取 intertidal zone macrobenthos carbon and nitrogen stable isotopes food sources and trophic levels study,sampling parts The technique of stable isotope analysis has been widely applied for the field of food web studies in recent years.However,few researches concerned about the impacts of different tissues used for analysis on the food web building.With the purpose of accuracy and comparability in the food web studies,the sampling tissues should be standardized.In this study,10macrobenthic species from the intertidal zone of the Yellow River Delta and Yantai were chosen as the target object to test the isotopic characteristic variations ofdelta~(13)C anddelta15 N at different body parts (whole bodies,muscles and gills).Our results show thatdelta~(13)C anddelta15 N values vary in different sampling tissues for most species,which indicates the different tissues used for analysis impact the food web building.To achieve a more accuracy and comparability result,suitable tissue should be primarily chosen for their food sources and trophic levels analysis.Namely,when the organism as predator,their muscles should first be chosen,for examples,adductor and foot muscles of mollusks,leg muscles of crabs and back muscles of fishes when the organism as prey, most of their tissues,except for indigestible part(for polychaetes,the digestive tract contents should be removed) should be chosen for analysis on food sources and trophic levels of the secondary consumer,for example,whole soft parts of mollusks,the mixture of muscles and gills of crabs.

食用蔬菜能吸收和积累微塑料 期刊论文 OAI收割
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者: 李连祯; 周倩; 尹娜; 涂晨; 骆永明

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微塑料生菜聚苯乙烯微球吸收积累健康风险 microplastics lettuce polystyrene microbeads uptake accumulation human health risk Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.

An analysis of first-time and repeat visitors to Korean national parks from 2007 and 2013 期刊论文 OAI收割
Journal of Mountain Science, 2017, 卷号: 14, 期号: 12, 页码: 2527-2539
作者: Yunseon CHOE; Michael A. SCHUETT; Kyu-Won SIM

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ThIs Paper ExamIned VIsiTors To Bukhansan NatiOnal Park In Korea Based On First-time And Repeat vIsitatiOn. Bukhansan NatiOnal Park Is Korea's Most Heavily vIsited NatiOnal Park With Annual vIsitatiOn Of Six milliOn VIsiTors In 2015. Data Used In ThIs Article Were Collected On-site In The Park In 2007 And 2013 By The Korea NatiOnal Park Service (Knps). The Study Variables Included Socio-demographics Travel Behavior Motivation And Satisfaction With Park Attributes Services And Facilities. Data Were Analyzed By Each Study Year For First-time And Repeat Visitors. Results Showed Significant Differences For Both First-time And Repeat Visitors.On Several Socio-demographic Travel Behavior And Motivation Variables Within Each Study Year. For Satisfaction With Park Attributes Facilities And Services First-time VisiTors Were Significantly Different From Repeat VisiTors On Seven Variables For Both Years. implicatiOns Are Given Regarding Programs And Services To Improve visiTor satisfactiOn And Sustain The Natural Cultural And HisTorical Resources At Bukhansan nAtional Park And Other nAtional Parks In Korea. Future Research Suggestions Are Provided To Learn More About visiTor Characteristics StAndards Of Quality And Preferences In The National Parks.

Molecular Weight Dependency of Crystallization and Melting Behavior of beta-Nucleated Isotactic Polypropylene 期刊论文 OAI收割
journal of polymer science part b-polymer physics, 2014, 卷号: 52, 期号: 19, 页码: 1301-1308
Lu, Ying; Wang, Qiao; Men, Yongfeng

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Compounds of isotactic polypropylene (iPP) and beta-nucleating agent were used to investigate the relationship between the development of beta phase and molecular weight in iPP under quiescent crystallization conditions by using wide angle X-ray diffraction and differential scanning calorimetry techniques. In all cases the dependency of the formation of beta phase in iPP on molecular weight of iPP at a defined crystallization temperature range was found. The iPP with high molecular weight possessed a wide range of crystallization temperature in inducing rich beta phase. However poor or even no beta phase was obtained for the samples with low molecular weight in the same range. In addition an upper critical crystallization temperature of producing dominant beta phase was found at 125 degrees C. Beyond this temperature a phenomenon of prevailing alpha phase became obvious. (C) 2014 Wiley Periodicals Inc.

Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells 期刊论文 OAI收割
PHARMAZIE, 2013, 卷号: 68, 期号: 4, 页码: 293-299
作者: Zeng, Guang-Zhi; Fan, Jun-Ting; Xu, Jun-Ju; Li, Yan; Tan, Ning-Hua

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2-Methyl-1,3,6-trihydroxy-9,10-anthraquinone (MTA) one of the major components isolated from the traditional Chinese medicine Rubia yunnanensis exhibited inhibitory activity on the proliferation of several human cancer cell lines. The results from an annexin V-FITC (fluoresein-5-isothiocyanate) apoptosis assay and DNA content analysis showed that MTA exerted cytotoxicity via apoptosis induction and G2/M cell cycle arrest in human cervical carcinoma HeLa cells. Further MTA was found to induce apoptosis of HeLa cells through the mitochondria-mediated pathway. It caused the translocation of Bax to the mitochondria and release of cytochrome c into the cytosol which caused the cleavage of caspase and poly(ADP-ribose) polymerase and finally triggered the apoptosis. Furthermore the p53/p21/Cdc2-cyclin B1 signaling was found related to the G2/M arrest caused by MTA. The over-expression of p21 and down-expression of cyclin B1 caused by MTA inactivated the Cdc2-cyclin B1 complex of G2/M checkpoint and finally caused the G2/M arrest in HeLa cells. This study demonstrated that MTA is a potential anti-cancer component of R. yunnanensis a folk anti-cancer herb used in Yunnan China.

Structural design and simulation analysis of ejector used in automotive power steering oil discharge equipment (EI CONFERENCE) 会议论文 OAI收割
2012 International Conference on Mechanical Engineering, Materials Science and Civil Engineering, ICMEMSCE 2012, August 18, 2012 - August 20, 2012, Harbin, China
Cheng K.; Zou H.; Kong X.; Tang J.

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The structure of ejector used in automotive power steering oil discharge equipment is designed in accordance with the theory of gas-liquid ejector.The two important parameters of the ejector are elected respectively to five values and then 25 ejector models are obtained by the permutations and combinations.The ejector's CFD model is created by Fluent module of Ansys then it is divided into a grid and analyzed. Analyse its pressure distribution and velocity vector which provides the optimal size for the manufacture of the ejectors and a theoretical basis for experiment. (2013) Trans Tech Publications Switzerland.

Simulation test system of CCD signal processor based on FPGA (EI CONFERENCE) 会议论文 OAI收割
2nd International Conference on Mechatronics and Applied Mechanics, ICMAM 2012, December 8, 2012 - December 9, 2012, Taiwan
作者: Zhang D.; Liu D.-B.; Zhang D.

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Simulation test system of CCD signal processor based on FPGA is proposed. Field-programmable gate array (FPGA) is applied as control processing core and used in the aerospace upgrade screening of industrial grade signal processor. The system functional composition and working principle were introduced in detail. Actually evaluation and testing system was completed with integrating the forty signal processor in a single board. The system has been successfully applied in the dynamic build-in screening test of industrial grade signal processor XRD98L63 the system work stably and reliability. Test result is zero on the nonconforming product rate satisfying the qualification that single batch screening nonconforming rate is less than 5% build-in screening of industrial grade components for the space load applications is implemented. (2013) Trans Tech Publications Switzerland.

Angle measurements with laser (EI CONFERENCE) 会议论文 OAI收割
2012 International Conference on Measurement, Instrumentation and Automation, ICMIA 2012, September 15, 2012 - September 16, 2012, Guangzhou, China
作者: Wang Y.; Wang Y.; Wang Y.; Wang Y.; Wang Y.

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Laser is widely used in angle measurements. This paper presents several frequently used methods of angle measurement that utilize laser including:autocollimation angle measurement angle measurement based on internal-reflection effect laser interference angle measurement angle measurement with ring laser and angle measurement with CCD. The principle actualities application situation superiority and defects of each method are discussed. (2013) Trans Tech Publications Switzerland.

Response spectrum 0.9-2.65 m of In0.82Ga0.18As detectors by two-step growth technique (EI CONFERENCE) 会议论文 OAI收割
2012 International Conference on Material Sciences and Manufacturing Technology, ICMSMT 2012, October 5, 2012 - October 6, 2012, Dalian, China
作者: Zhang T.

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InP/In0.82Ga0.18As/InP heterostructure used for infrared detector were grown on (100) S-doped InP substrates using two-step growth technique by low temperature metal-organic chemical vapor deposition. The growth was performed using TMIn TMGa AsH3 and PH3 as growth precursors in a horizontal reactor. The substrates on a graphite susceptor were heated by inductively coupling RF power their temperatures were detected by a thermocouple and the reactor pressure was kept at 10000 Pa. The growth structure of detector included In0.82Ga0.18As buffer with the thickness of 100 nm In0.82Ga0.18As absorption layer with the thickness of 2.8 m and the InP cap with the thickness of 0.8 m. The planar type of p-i-n detector was fabricated by Zn diffusion. The properties of In0.82Ga0.18As detector were studied the curves of the I-V characteristics the range of response spectrum and the detectivity (D*) were obtained. (2013) Trans Tech Publications Switzerland.