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Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry 期刊论文  OAI收割
Thin Solid Films, 2011, 卷号: 519, 期号: 9, 页码: 2768-2771
作者:  
Niu Y(牛宇);  Zhuang J;  Liu L(刘丽);  Yan XY;  Jin G(靳刚)
收藏  |  浏览/下载:25/0  |  提交时间:2012/04/01
Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry 会议论文  OAI收割
5th International Conference on Spectroscopic Ellipsometry, Albany, NY, MAY 23-29, 2010
作者:  
Liu L(刘丽);  Jin G(靳刚);  Niu Y(牛宇)
收藏  |  浏览/下载:29/0  |  提交时间:2012/04/01
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文  OAI收割
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
作者:  
Wang X.;  Wang X.;  Wang X.
收藏  |  浏览/下载:40/0  |  提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper  FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore  red shift of the FITC fluorescence dye emission peak was observed on different fiber surface  exhibiting different interaction between FITC- labeled antibody and surface. Finally  under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.