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Optical microscopy: advances and applications 期刊论文  OAI收割
FRONTIERS IN PHYSICS, 2023, 卷号: 11
作者:  
Dan, Dan
  |  收藏  |  浏览/下载:12/0  |  提交时间:2024/01/10
Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope 期刊论文  OAI收割
SCIENCE CHINA-CHEMISTRY, 2016, 卷号: 59, 期号: 11, 页码: 1519-1524
作者:  
Wang, SP;  Deng, SH;  Cai, XQ;  Hou, SG;  Li, JJ
收藏  |  浏览/下载:50/0  |  提交时间:2017/03/02
Mechanism of STED microscopy and analysis of the factors affecting resolution (EI CONFERENCE) 会议论文  OAI收割
6th International Symposium on Precision Engineering Measurements and Instrumentation, August 8, 2010 - August 11, 2010, Hangzhou, China
Yang P.; Ai H.
收藏  |  浏览/下载:22/0  |  提交时间:2013/03/25
Stimulated emission depletion (STED) microscopy exploits nonlinear saturable optical transition of fluorescent molecules  allowed to overcome Abbe's diffraction-limit and provides diffraction-unlimited resolution in far-field optical microscopy. We elaborate the mechanism of STED and the conditions of depletion. The formula of STED microcopy resolution is deduced through effective point spread function (E-PSF). The STED system resolution is mainly dominated by the quality of the fluorescence depletion patterns in the focal plane. The depletion pattern is mainly affected by STED beam intensity  polarization  phase plate  primary aberrations  STED pulse shape  pulse duration and delay time. In this paper  we found related models and simulate the relationship between the depletion patterns and the parameters  and put forward effective approach to enhance the system resolution. 2010 SPIE.  
Introduction to Theories of Several Super-resolution Fluorescence Microscopy Methods and Recent Advance in The Field 期刊论文  OAI收割
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2009, 卷号: 36, 期号: 12, 页码: 1626-1634
Lue ZhiJian; lu jingze; 陆敬泽; Wu YaQiong; Chen Liangyi; 陈良怡
收藏  |  浏览/下载:102/0  |  提交时间:2013/12/25
Investigation of the influence of the aberration induced by a plane interface on STED microscopy 期刊论文  OAI收割
opt. express, 2009, 卷号: 17, 期号: 3, 页码: 1714, 1725
Deng Suhui; Liu Li; 程亚; 李儒新; 徐至展
收藏  |  浏览/下载:962/90  |  提交时间:2009/09/18