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Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry 期刊论文  OAI收割
BIOTECHNOLOGY LETTERS, 2020
作者:  
Hu, Lei;  Chen, Xueran;  Chen, Meng;  Fang, Jinman;  Nie, Jinfu
  |  收藏  |  浏览/下载:110/0  |  提交时间:2020/11/30
Recognition and transmembrane delivery of bioconjugated Fe2O3@Au nanoparticles with living cells 期刊论文  OAI收割
nanoscale, 2010, 卷号: 2, 期号: 2, 页码: 269-276
Sun LL; Wang JE; Wang ZX
收藏  |  浏览/下载:18/0  |  提交时间:2012/06/07
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文  OAI收割
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
作者:  
Wang X.;  Wang X.;  Wang X.
收藏  |  浏览/下载:40/0  |  提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper  FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore  red shift of the FITC fluorescence dye emission peak was observed on different fiber surface  exhibiting different interaction between FITC- labeled antibody and surface. Finally  under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.