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	食用蔬菜能吸收和积累微塑料期刊论文 OAI收割科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934 作者: 李连祯; 周倩; 尹娜; 涂晨; 骆永明 \| 收藏 \| 浏览/下载：146/0 \| 提交时间：2020/06/17 微塑料生菜聚苯乙烯微球吸收积累健康风险 microplastics lettuce polystyrene microbeads uptake accumulation human health risk Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.
	Hyper-production of C-13-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption 期刊论文 OAI收割 biochemical engineering journal, 2011, 卷号: 53, 期号: 3, 页码: 292-296 作者: Yue, Xiangguo; Zhang, Wei; Deng, Maicun 收藏 \| 浏览/下载：21/0 \| 提交时间：2015/11/12 Vitis vinifera C-13-labeled trans-resveratrol Secondary metabolites Plant cell tissue Culture engineering Metabolic control Adsorption
	Root and vascular tissue-specific expression of glycine-rich protein atgrp9 and its interaction with atcad5, a cinnamyl alcohol dehydrogenase, in arabidopsis thaliana 期刊论文 iSwitch采集 Journal of plant research, 2007, 卷号: 120, 期号: 2, 页码: 337-343 作者: Chen, An-Ping; Zhong, Nai-Qin; Qu, Zhan-Liang; Wang, Fang; Liu, Ning 收藏 \| 浏览/下载：22/0 \| 提交时间：2019/05/10 Cinnamyl alcohol dehydrogenase Glycine-rich protein Plant cell wall Vascular tissue
	Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors 期刊论文 OAI收割 Journal of Chemical Technology and Biotechnology, 1998, 期号: 3, 页码: 227-234 Xu, J. F.; Xie, J.; Han, A. M.; Feng, P. S.; Su, Z. G. 收藏 \| 浏览/下载：21/0 \| 提交时间：2013/11/15 Rhodiola sachalinensis salidroside plant cell culture aggregates air-lift reactor oxygen transfer coefficient plant-cell cultures tissue-cultures growth accumulation root