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Chinese Academy of Sciences Institutional Repositories Grid
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Deterioration of air quality associated with the 2020 US wildfires 期刊论文  OAI收割
SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 卷号: 826, 页码: 11
作者:  
Filonchyk, Mikalai;  Peterson, Michael P. P.;  Sun, Dongqi
  |  收藏  |  浏览/下载:42/0  |  提交时间:2022/09/21
Deterioration of air quality associated with the 2020 US wildfires 期刊论文  OAI收割
SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 卷号: 826, 页码: 11
作者:  
Filonchyk, Mikalai;  Peterson, Michael P. P.;  Sun, Dongqi
  |  收藏  |  浏览/下载:36/0  |  提交时间:2022/09/21

Exploring the causal relationship between urbanization and air pollution: Evidence from China

期刊论文  OAI收割

SUSTAINABLE CITIES AND SOCIETY, 2022, 卷号: 80, 页码: 12
作者:  
Liu, Haimeng;  Cui, Weijia;  Zhang, Mi
  |  收藏  |  浏览/下载:22/0  |  提交时间:2022/09/21

Exploring the causal relationship between urbanization and air pollution: Evidence from China

期刊论文  OAI收割

SUSTAINABLE CITIES AND SOCIETY, 2022, 卷号: 80, 期号: 2022, 页码: 12
作者:  
Liu, Haimeng;  Cui, Weijia;  Zhang, Mi
  |  收藏  |  浏览/下载:18/0  |  提交时间:2022/09/21
Investigation on the difference of PM2.5 transport flux between the North China Plain and the Sichuan Basin 期刊论文  OAI收割
ATMOSPHERIC ENVIRONMENT, 2022, 卷号: 271
作者:  
Zhang, Zhida;  Wang, Xiaoqi;  Cheng, Shuiyuan;  Guan, Panbo;  Zhang, Hanyu
  |  收藏  |  浏览/下载:20/0  |  提交时间:2022/12/23
High-resolution simulation of wintertime fossil fuel CO2 in Beijing, China: Characteristics, sources, and regional transport 期刊论文  OAI收割
ATMOSPHERIC ENVIRONMENT, 2019, 卷号: 198, 页码: 226-235
作者:  
Feng, Tian;  Zhou, Weijian;  Wu, Shugang;  Niu, Zhenchuan;  Cheng, Peng
  |  收藏  |  浏览/下载:54/0  |  提交时间:2020/06/23
Comparisons of IL-8, ROS and p53 responses in human lung epithelial cells exposed to two extracts of PM2.5 collected from an e-waste recycling area, China 期刊论文  OAI收割
ENVIRONMENTAL RESEARCH LETTERS, 2011, 卷号: 6, 期号: 2, 页码: 24013
作者:  
Yang, Fangxing;  Jin, Shiwei;  Xu, Ying;  Lu, Yuanan;  Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, Hangzhou 310003, Zhejiang, Peoples R China
  |  收藏  |  浏览/下载:21/0  |  提交时间:2011/12/22
Il-8  Ros  P53  Pm2.5  A549 Cells  E-waste  
A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE) 会议论文  OAI收割
International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009, Beijing, China
作者:  
Song K.-F.;  Li J.-L.;  Zhang W.-L.;  Wang Y.-L.
收藏  |  浏览/下载:37/0  |  提交时间:2013/03/25
In the device  a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source  and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system  so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least  the sample fluorescence through the integrating sphere and come to the grating monochromator  The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device  SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process  signal-to-noise ratio(SNR) will be improved  also adjust delay time  ampling frequency and sampling number arbitrarily. By testing  the sensitivity is 10-12mol/L(substance marked by Eu3+)  examination repeat is &le2.5%  examination linearity is from 10 -8mol/L to 10-12mol/L  correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody  and solve the tumor  genetic variation  the virus protein detection. 2009 SPIE.  
龙须菜选育品系和野生型及相关种鉴定的方法 专利  OAI收割
专利类型: 发明, 专利号: CN200410021012.9, 申请日期: 2005-07-20, 公开日期: 2005-07-20
段德麟; 李文红; 胡自民
收藏  |  浏览/下载:62/0  |  提交时间:2014/08/04
1.龙须菜选育品系和野生型及相关种鉴定的方法  提取缓冲液的组成为:100mmolL-1Tris.HCl  (2)PCR扩增:龙须菜ITS区扩增的特异引物P1  P2:5’GGGATCCATATGCTTAAGTTCAGCGGGT3’  利用该引物对步骤(1)提取的DNA为模板  (3)PCR产物纯化  (4)DNA序列测定:纯化后的DNA进行序列测定  (5)DNA序列数据分析:待分析的rDNAITS区的序列一经测定  建立用于鉴别龙须菜种内和种间差异的RAPD和ISSR特异指纹图谱  2)RAPD和ISSR引物的合成  3)根据扩增条带的多态性  其特征在于:龙须菜的rDNAITS全序列的获取  pH8.0  P2的序列分别为:P1:5’GGGATCCGTTTCCGTAGGTGAACCTGC3’  位于26S上  对总DNA进行PCR扩增反应  确立rDNAITS区  用对位排列软件ClustalW进行对位排列  步骤如下:1)总DNA提取  从大量随机引物中筛选出能稳定的扩增出特征条带的引物  构建龙须菜选育品系及野生型和相关种的RAPD和ISSR特异指纹图谱。  步骤如下:(1)总DNA提取:取新鲜健康的藻体材料  50-100mmolL-1EDTA  位于18S上  包括ITS1和5.8S区的全序列  并辅以人工校对  用无菌水处理后  0.2-0.5MNaCl  用系统进化分析各样品DNA序列与数据库中各个序列间的差异  在液氮种研磨成粉末  5%β-巯基乙醇  提取总DNA  0.2%PVP-40  1.0-2.5%SDS  提取所用的KAc浓度为4.0-5.0M  pH值范围在5.2-7.5