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Chinese Academy of Sciences Institutional Repositories Grid
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浏览/检索结果: 共16条,第1-10条 帮助

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Ratiometric fluorescent nanosensor for dosage-sensitive visual discrimination of glucose based on electron transfer mechanism 期刊论文  OAI收割
MICROCHEMICAL JOURNAL, 2020, 卷号: 158
作者:  
Zhang, Na;  Shen, Yujun;  Pang, Gaozong;  Chu, Suyun;  Han, Wei
  |  收藏  |  浏览/下载:85/0  |  提交时间:2020/11/30
一种纯化FITC标记的虾白斑综合症病毒(WSSV)的方法 专利  OAI收割
专利类型: 发明, 专利号: CN201310302179.1, 申请日期: 2013-12-25, 公开日期: 2013-12-25
作者:  
谢世筠;  相建海
收藏  |  浏览/下载:88/0  |  提交时间:2014/08/04
Apoptosis induction and G2/M arrest of 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone from Rubia yunnanensis in human cervical cancer HeLa cells 期刊论文  OAI收割
PHARMAZIE, 2013, 卷号: 68, 期号: 4, 页码: 293-299
作者:  
Zeng, Guang-Zhi;  Fan, Jun-Ting;  Xu, Jun-Ju;  Li, Yan;  Tan, Ning-Hua
收藏  |  浏览/下载:40/0  |  提交时间:2015/05/14
2-Methyl-1,3,6-trihydroxy-9,10-anthraquinone (MTA)  one of the major components isolated from the traditional  Chinese medicine Rubia yunnanensis  exhibited inhibitory activity on the proliferation of several  human cancer cell lines. The results from an annexin V-FITC (fluoresein-5-isothiocyanate) apoptosis assay  and DNA content analysis showed that MTA exerted cytotoxicity via apoptosis induction and G2/M cell cycle  arrest in human cervical carcinoma HeLa cells. Further  MTA was found to induce apoptosis of HeLa cells  through the mitochondria-mediated pathway. It caused the translocation of Bax to the mitochondria and  release of cytochrome c into the cytosol  which caused the cleavage of caspase and poly(ADP-ribose)  polymerase and finally triggered the apoptosis. Furthermore  the p53/p21/Cdc2-cyclin B1 signaling was  found related to the G2/M arrest caused by MTA. The over-expression of p21 and down-expression of  cyclin B1 caused by MTA inactivated the Cdc2-cyclin B1 complex of G2/M checkpoint and finally caused  the G2/M arrest in HeLa cells. This study demonstrated that MTA is a potential anti-cancer component of  R. yunnanensis  a folk anti-cancer herb used in Yunnan  China.  
Core-shell plasmonic nanostructures to fine-tune long "Au nanoparticle-fluorophore" distance and radiative dynamics 期刊论文  OAI收割
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2013, 卷号: 421, 页码: 101-108
作者:  
Huang, Yi-Fan;  Ma, Kai-Hong;  Kang, Kai-Bin;  Zhao, Min;  Zhang, Ze-Ling
  |  收藏  |  浏览/下载:27/0  |  提交时间:2019/04/09
Evaluation of three tracers for labeling distal cerebrospinal fluidcontacting neurons 期刊论文  OAI收割
NEUROSCIENCE BULLETIN, 2013, 卷号: 29, 期号: 5, 页码: 576-580
作者:  
Zhou Fang;  Wang Jiayou;  Zhang Hongxing;  Liu He;  Zhao Guangping
  |  收藏  |  浏览/下载:25/0  |  提交时间:2021/02/02
一种快速检测自然水样中赤潮藻的试剂盒 专利  OAI收割
专利类型: 发明, 专利号: CN201010261804.9, 申请日期: 2012-03-14, 公开日期: 2012-03-14
王广策; 张宝玉; 陈国福
收藏  |  浏览/下载:32/0  |  提交时间:2014/08/04
Synthesis and characterization of amphiphilic block polymers with amino groups and their conjugates with folic acid and fluorescent probes 期刊论文  OAI收割
POLYMER INTERNATIONAL, 2011, 卷号: 60, 期号: 8, 页码: 1269-1276
作者:  
Mo, Guojun;  Yue, Jun;  Ma, Ping'an;  Chen, Xuesi;  Huang, Yubin
  |  收藏  |  浏览/下载:17/0  |  提交时间:2019/04/09
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文  OAI收割
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
作者:  
Wang X.;  Wang X.;  Wang X.
收藏  |  浏览/下载:38/0  |  提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper  FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore  red shift of the FITC fluorescence dye emission peak was observed on different fiber surface  exhibiting different interaction between FITC- labeled antibody and surface. Finally  under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.  
Development of a gel monolithic column polydimethylsiloxane microfluidic device for rapid electrophoresis separation 期刊论文  OAI收割
TALANTA, 2006, 卷号: 69, 期号: 1, 页码: 226-231
作者:  
Zeng, HL;  Li, HF;  Wang, X;  Lin, JM
收藏  |  浏览/下载:31/0  |  提交时间:2015/10/15
Development of a highly sensitive, second antibody format chemiluminescence enzyme immunoassay for the determination of 17 beta-estradiol in wastewater 期刊论文  OAI收割
ANALYTICA CHIMICA ACTA, 2006, 卷号: 558, 期号: 2015-01-02, 页码: 290-295
作者:  
Zhao, LX;  Lin, JM;  Li, ZJ;  Ying, XT
收藏  |  浏览/下载:27/0  |  提交时间:2015/10/15